Mepe (NM_053172) Mouse Untagged Clone

CAT#: MC215851

Mepe (untagged) - Mouse matrix extracellular phosphoglycoprotein with ASARM motif (bone) (Mepe), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mepe
• Synonyms: Of45
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC215851 representing NM_053172
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACGCCAGAGGGCCTCATGAAGATGCAGGCTGTGTCTGTTGGACTGCTCCTCTTCAGTATGACCTGGG
  CGGCACCAATGCCGAATGAAGACAGGAGCAGCTGCGGCAATCAAGACAGCATTCACAAGGACTTGGCAGC
  ATCTGTGTATCCTGATCCCACGGTGGATGAAGGCACAGAGGATGGGCAAGGTGCTCTCCTTCACCCGCCT
  GGCCAGGACAGGTATGGTGCTGCCCTCCTCAGAAATATCACGCAGCCTGTAAAGAGTCTAGTGACTGGGG
  CCGAACTACGGAGGGAAGGAAACCAGGAGAAGAGACCTCAGAGTGTTCTAAGCGTAATTCCAGCAGATGT
  CAATGATGCTAAAGTCTCCTTAAAAGACATAAAGAATCAAGAGAGTTATCTGCTAACCCAGAGCAGCCCG
  GTCAAAAGCAAACACACCAAACACACCCGCCAGACCCGACGGAGCACTCACTACCTGACACATCTCCCAC
  AGATCAAGAAGACTCCCAGTGACCTTGAAGGCAGTGGCTCCCCAGATCTTCTAGTGAGGGGAGATAATGA
  TGTCCCCCCTTTCAGTGGAGATGGGCAACATTTTATGCACATTCCTGGCAAAGGAGGTGCTGGGTCTGGT
  CCTGAAAGCTCAACTAGTCGCCCCCTCTCAGGCTCCAGCAAAGCTGAAGTTATTGACCCACATATGAGTG
  GACTAGGCTCTAATGAGATCCCGGGGAGAGAAGGACATGGTGGCAGTGCCTATGCAACCAGAGACAAAGC
  TGCACAGGGGGCAGGCTCTGCAGGTGGGAGCCTTGTGGGGGGCAGCAATGAAATCACAGGCAGCACCAAT
  TTCAGGGAACTCCCCGGAAAAGAAGGAAACAGAATTAATGCCGGCAGCCAAAATGCTCATCAAGGGAAAG
  TAGAATTTCACTATCCACAAGTGGCCTCGAGAGAAAAGGTAAAGGGGGGCGTGGAGCATGCAGGGAGAGC
  TGGTTACAACGAAATCCCCAAGAGCAGCAAAGGTAGCTCTAGCAAAGATGCAGAAGAGTCCAAAGGGAAC
  CAATTAACCTTGACTGCAAGCCAAAGATTTCCAGGTAAAGGCAAAAGCCAGGGCCCTGCTCTGCCCTCTC
  ACAGTCTTAGTAATGAGGTTAAAAGTGAAGAAAACCATTATGTGTTCCATGGACAAAATAATCTTACACC
  GAATAAAGGGATGTCACAGCGGAGAGGCTCCTGGCCTTCGAGAAGACCCAATTCCCACAGGCGCGCTAGC
  ACCCGCCAAAGAGACAGCAGCGAGTCGTCATCCAGTGGGAGTTCTAGTGAGAGTCATGGTGACTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_053172
• Insert Size: 1326 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_053172.2, NP_444402.2
• RefSeq Size: 1682 bp
• RefSeq ORF: 1326 bp
• Locus ID: 94111
• UniProt ID: Q8K4L6
• Cytogenetics: 5 E5
• Gene Summary: Regulates renal phosphate and uric acid excretion (PubMed:26051469). Regulates bone mineralization by osteoblasts and cartilage mineralization by chondrocytes (PubMed:11414762, PubMed:12421822, PubMed:15843468, PubMed:22766095). Regulates the mineralization of the extracellular matrix of the craniofacial complex, such as teeth, bone and cartilage (PubMed:26927967). Increases dental pulp stem cell proliferation (By similarity).[UniProtKB/Swiss-Prot Function]