Gapdhs (NM_008085) Mouse Untagged Clone

CAT#: MC215785

Gapdhs (untagged) - Mouse glyceraldehyde-3-phosphate dehydrogenase, spermatogenic (Gapdhs), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Gapdhs
• Synonyms: Gapd-s; Gapds
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC215785 representing NM_008085
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGAGACGTGACGTGGTCCTTACCAATGTTACTGTTGTCCAGCTACGGCGGGACCGATGCCCATGCC
  CATGCCCATGTCCATGCCCATGCCCTGTGATCAGACCACCTCCACCCAAGGTTGAGGATCCACCACCCAC
  GGTTGAAGAACAGCCACCGCCACCGCCGCCGCCACCTCCACCTCCACCACCACCTCCTCCTCCTCCTCCA
  CCCCAGATAGAGCCAGACAAGTTTGAAGAGGCTCCCCCTCCCCCTCCCCCTCCTCCTCCTCCTCCCCCTC
  CCCCTCCCCCACCACTCCAAAAGCCAGCTAGAGAGCTGACAGTGGGTATCAATGGATTTGGACGCATTGG
  TCGTCTGGTGCTGCGAGTCTGCATGGAGAAGGGCATTAGGGTGGTAGCAGTGAATGACCCATTCATTGAT
  CCAGAATACATGGTTTACATGTTCAAATATGACTCCACACATGGTAGATACAAAGGAAACGTGGAACATA
  AGAATGGACAACTAGTTGTGGACAACCTTGAGATCAACACGTACCAGTGCAAAGACCCTAAAGAAATCCC
  CTGGAGCTCTATAGGGAATCCCTACGTGGTGGAGTGTACAGGCGTCTATCTGTCCATCGAGGCAGCTTCG
  GCACATATTTCATCTGGTGCCAGGCGTGTGGTGGTCACTGCACCCTCCCCCGATGCACCCATGTTTGTCA
  TGGGAGTGAACGAGAAGGACTATAACCCTGGCTCTATGACCATTGTCAGCAATGCATCCTGTACCACCAA
  CTGCCTGGCTCCTCTCGCCAAGGTTATTCATGAAAACTTCGGGATCGTGGAAGGGCTAATGACCACAGTC
  CATTCCTACACAGCCACTCAGAAGACAGTGGATGGGCCATCAAAGAAGGACTGGCGAGGTGGCCGCGGCG
  CTCACCAAAACATCATCCCATCGTCCACTGGGGCTGCCAAGGCTGTAGGCAAAGTCATCCCAGAGCTCAA
  AGGGAAGCTAACAGGAATGGCATTCCGGGTGCCAACCCCAAACGTGTCAGTTGTGGACCTGACCTGCCGC
  CTGGCCAAGCCTGCTTCTTACTCGGCTATCACGGAGGCTGTGAAAGCTGCAGCCAAGGGACCTTTGGCTG
  GCATCCTTGCTTACACAGAGGACCAGGTGGTCTCCACGGACTTTAACGGCAATCCCCATTCTTCCATCTT
  TGATGCTAAGGCTGGAATTGCCCTCAATGACAACTTCGTGAAGCTTGTTGCCTGGTACGACAACGAATAT
  GGCTACAGTAACCGAGTGGTCGACCTCCTCCGCTACATGTTTAGCCGAGAGAAGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_008085
• Insert Size: 1317 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008085.2, NP_032111.1
• RefSeq Size: 1476 bp
• RefSeq ORF: 1317 bp
• Locus ID: 14447
• UniProt ID: Q64467
• Cytogenetics: 7 B1
• Gene Summary: May play an important role in regulating the switch between different pathways for energy production during spermiogenesis and in the spermatozoon. Required for sperm motility and male fertility.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) contains an alternate first exon compared to variant 1. The resulting isoform (2) has a shorter and distinct N-terminus compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.