Gcm1 (NM_008103) Mouse Untagged Clone

CAT#: MC215698

Gcm1 (untagged) - Mouse glial cells missing homolog 1 (Drosophila) (Gcm1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Gcm1
• Synonyms: Gcm-rs2; Gcm1-rs1; GCMa; glide
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC215698 representing NM_008103
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAACTGGACGACTTTGATCCTGAAGACAAAGAGATACTGAGCTGGGACATTAACGATGTGAAACTGC
  CTCAGAACGTGAAAACGACTGACTGGTTCCAGGAGTGGCCGGACTCCTACGTGAAACACATCTACAGCTC
  GGACGACAGGAACGCACAGCGCCACCTGAGCAGCTGGGCCATGCGCAACACCAACAACCACAACTCCCGG
  ATCCTCAAGAAGTCATGCCTGGGGGTAGTGGTGTGCAGCAGGGACTGCTCCACAGAGGAAGGCCGCAAGA
  TTTACCTGAGACCCGCCATCTGTGACAAAGCCAGACAGAAGCAGCAGAGGAAAAGCTGTCCCAACTGCAA
  TGGACCCCTGAAGCTTATTCCCTGCCGAGGCCACGGCGGCTTCCCGGTCACCAACTTCTGGAGGCACGAC
  GGACGCTTCATCTTTTTCCAGTCCAAAGGCGAGCATGATCATCCCAGGCCGGAGACCAAGCTGGAAGCAG
  AGGCAAGAAGAGCCATGAAGAAAGTGCACATGGCATCTGCCTCCAACTCCTTACGGATGAAGGGGAGACC
  AGCAGCGAAGGCGCTTCCTGCTGAAATCCCGAGTCAGGGAAGTTTACCTTTAACTTGGTCATTCCAGGAA
  GGCGTCCAACTGCCCGGCACTTACAGCACACCTTTAATAGCTAACGCCCCCCAGCAGAACTCCCTGAATG
  ATTGCTTATCCTTCCCCAAGAGTTATGATTTGGGGGGAAGCACTGAGCTGGAAGATCCAACTTCCACCTT
  AGATTCCATGAAGTTCTATGAGAGATGCAAATTCTCCAGCAGTAGGATCTACGGCAGTGAAGAGCAGTTT
  CAGCCTCCTGTCCCTGGGACGTATGGAGACTACGAAGACCTGCAAACTTGGAATAAAAATGTTGCCTTAG
  GGAGAAATCCCTCCGATGACATCTACTATCCAGCCTATCCTCTGCCTGTGGCCAGCTGGCCCTACGACTA
  CTTTCCCTCCCAGAACTCTTTGGAGCACTTACCCCAGCAAGTTCCATCAGAACCCCCTGCAGCTCAACCA
  GGCTGTCATCCCTTGTGGTCCAATCCGGGAGGTGAGCCTTACGAAGAGAAAGTATCTGTGGATCTGAGCA
  GCTATGTGCCCTCCCTCACGTACCACCCACCTCAGCAGGATCCCTTCCTGCTCACCTATGGCTCTCCTAC
  TCAGCAGCAACATGCACTGCCCGGCAAGAGCAACAGGTGGGATTTTGACGAAGAGATGGCATGCATGGGC
  TTAGATCACTTCAACAATGAGATGCTCCTAAACTTCTGTTCTTTAAGATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_008103
• Insert Size: 1311 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC066866, AAH66866
• RefSeq Size: 2034 bp
• RefSeq ORF: 1311 bp
• Locus ID: 14531
• UniProt ID: P70348
• Cytogenetics: 9 43.49 cM
• Gene Summary: Transcription factor that is necessary for placental development (PubMed:8962155). Involved in the control of expression of placental growth factor (PGF) and other placenta-specific genes. Binds to the trophoblast-specific element 2 (TSE2) of the aromatase gene enhancer. Binds to the SYDE1 promoter. Has a central role in mediating the differentiation of trophoblast cells along both the villous and extravillous pathways in placental development (By similarity).[UniProtKB/Swiss-Prot Function]