Rsph3b (NM_001083945) Mouse Untagged Clone

CAT#: MC215460

Rsph3b (untagged) - Mouse radial spoke 3B homolog (Chlamydomonas) (Rsph3b), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rsph3b
• Synonyms: Rshl2b
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC215460 representing NM_001083945
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACAGACCGTAACCCTCGGACAGCCGAGGCCTCAGGCCTCTACACCTATTCCAGCAGGCCCCGGGCCG
  TGGCTTGCCAGCGCCGCCGCCACCGAGACAGCATTTTGCAGCCAGTTGAAGAACCTATGAGTTACGGGAA
  CATCATGTATGACAGAAGGGTGATCCGAGGCAACACCTATGCACTGCCCACAGGACAGGTGCCTGGACAG
  CCTGATCCTCTAGAGCTTCAGCGACAGCAGCAGGCCAGGAGAAGGGCTCTTGCCAGAAAACGAGCCCAGG
  AGCAGCTCAAACCACGAACGCCTGAGCCCGTGGAGGGCAGAAAGCATGTGGACATACAAACAGAATTATA
  CCTTGAAGAAATTGCTGATCGCATAGTTGAAGTCGACATGGAATGCCAGACAGATGCGTTTCTGGACAGA
  CCACCCACACCCCTCTTCATTCCTGCCAAAACTGGCAAAGATGTGGCCACTCAGATCCTGGGAGGAGAGC
  TTTTCGACTTTGACCTTGAAGTCAAGCCCATGTTAGAGGTTTTGGTGGGGAAGACGATTGAACAGTCCCT
  TCTAGAGGTGATGGAAGAGGAGGAGCTGGCCAACCTGCGGGCCAGGCAGTACGCATATGAAGAGATAAGG
  AACGTGGAGCTGGCCGAAGTGCAGCGACTGGAAGAGCAAGAGAGAAGGCACCGAGAAGAAAAGGAGCGCC
  GTAAGAAACAGCAGTGGGAGATTGTCCATAAGCGGAATGAGACGTTACAGAAGATCTCAGCTCTGATATT
  CGCTCGGCAGTACCTGGCTAACCTCCTCCCGTCTGTCTTTGACAAACTCAGGAATAGTGGCTTCTTTTAT
  GATCCAATTGAAAGAGATATTGAGGTAGGATTCCTCCCATGGCTAATGAATGAAGTTGAAAAATCCATGG
  AACACAGCATGGTGGGAAGAACTGTGCTTGACATGTTAATTCGCGACGTGGTTGAAAGAAGGATCAACGA
  TTACGAGCACAAGGAAGCCATGCCGCCTGGTCAGAAGACAAATGTGATTAATGGTCCTAACACAGTGACA
  GATCCCTCGGTGACCACATTGCACACGCAGAAGCCAGTTTTAGACAGAGTCTCCTCACAGCCAGCACCAT
  CCCAAGAAAGGAAGCCTGTGGAAGAGGGGGGACACCTTATGGCAGAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001083945
• Insert Size: 1170 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001083945.1, NP_001077414.1
• RefSeq Size: 1614 bp
• RefSeq ORF: 1170 bp
• Locus ID: 100037282
• UniProt ID: Q9DA80
• Cytogenetics: 17 A1
• Gene Summary: Functions as a protein kinase A-anchoring protein that scaffolds the cAMP-dependent protein kinase holoenzyme. May serve as a point of convergence for MAPK and PKA signaling in cilia (By similarity).[UniProtKB/Swiss-Prot Function]