Alkbh5 (NM_172943) Mouse Untagged Clone

CAT#: MC214261

Alkbh5 (untagged) - Mouse alkB, alkylation repair homolog 5 (E. coli) (Alkbh5), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Alkbh5
• Synonyms: Abh5; AW050020; AW215868; E130207K11; Ofoxd
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC214261 representing NM_172943
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCCGCCAGCGGCTACACCGACCTGCGGGAGAAGCTCAAGTCCATGACGTCCCGGGACAACTACA
  AGGCGGGCAGTCGGGAGGCCGCCGCCGCTGCGGCCGCCGCCGTGGCTGCCGCTGCCGCTGCCGCGGCCGC
  CGCTGAGCCTTACCCGGCGTCCGGGACCACCAAGCGGAAATACCAGGAGGACTCGGACCCCGAGCGCAGC
  GACTACGAGGAGCATCAGTTGCAGAAGGAGGAAGAGGCGCGCAAGGTGAAGAGCGGCATCCGGCAGATCC
  GGCTCTTCAGTCAGGATGAGTGCTCCAAGATCGAGGCCCGCATCGATGAGGTGGTGTCCCGCGCCGAGAA
  GGGCCTGTACAACGAGCACACGGTGGACCGGGCCCCCCTGCGCAACAAGTACTTCTTCGGCGAGGGCTAC
  ACGTACGGGGCCCAGCTGCAGAAGCGCGGGCCGGGCCAGGAGCGCCTCTACCCGCCGGGCGACGTCGACG
  AGATCCCGGACTGGGTGCATCAGCTGGTGATCCAGAAGCTGGTGGAGCACCGCGTCATCCCCGAGGGCTT
  CGTCAACAGCGCGGTCATCAACGACTACCAGCCCGGCGGCTGCATCGTGTCCCACGTTGACCCCATCCAC
  ATCTTCGAGCGCCCCATCGTGTCCGTGTCTTTCTTCAGCGACTCGGCACTTTGCTTCGGCTGCAAGTTCC
  AGTTCAAGCCCATCCGGGTGTCGGAACCTGTGCTTTCTCTGCCGGTGCGCAGGGGGAGCGTGACTGTGCT
  CAGTGGGTATGCTGCTGATGAAATCACTCACTGCATACGGCCTCAGGACATTAAGGAACGCCGGGCGGTC
  ATCATTCTCAGGAAGACAAGATTAGATGCACCGCGATTGGAAACAAAATCCCTGAGCAGCTCCACATTGC
  CACCCAGCTATGCTTCAGATCGCCTGTCAGGAAACACCAGAGACCCTGCGCTGAAACCCAAAAGGTCCCA
  CCGCAAGGCAGACCCTGATGCTGCCCACAGGCCCCGGATCCTGGAAATGGACAAAGAAGAAAACCGGCGG
  TCTGTGCTCCTGCCCACACACCGGCGGAGGGGGAGTTTTAGCTCTGAGAACTATTGGCGCAAATCCTATG
  AGTCCTCGGAAGATTGCCCAGAGGCAGCCAGCAGCCCCACCCGCAAGGTGAAGATGAGGAGACACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_172943
• Insert Size: 1188 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172943.4, NP_766531.2
• RefSeq Size: 6025 bp
• RefSeq ORF: 1188 bp
• Locus ID: 268420
• UniProt ID: Q3TSG4
• Cytogenetics: 11 B2
• Gene Summary: Dioxygenase that demethylates RNA by oxidative demethylation: specifically demethylates N(6)-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes (PubMed:29279410). Can also demethylate N(6)-methyladenosine in single-stranded DNA (in vitro) (By similarity). Requires molecular oxygen, alpha-ketoglutarate and iron (By similarity). Demethylation of m6A mRNA affects mRNA processing and export (By similarity). Required for the late meiotic and haploid phases of spermatogenesis by mediating m6A demethylation in spermatocytes and round spermatids: m6A demethylation of target transcripts is required for correct splicing and the production of longer 3' UTR mRNAs in male germ cells (PubMed:23177736, PubMed:29279410).[UniProtKB/Swiss-Prot Function]