Tmem231 (NM_001033321) Mouse Untagged Clone

CAT#: MC212991

Tmem231 (untagged) - Mouse transmembrane protein 231 (Tmem231), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tmem231
• Synonyms: 4932417I16Rik; Gm178
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC212991 representing NM_001033321
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCTGTACCATCTCTTCTCTCACCCAATCGAGCGCGCTTACCGCGCGGGGCTCTGCTCCAAGGCCG
  CGCTCTTCCTGCTGTTGACCACCGCGCTCACCTACATCCCCCCGCTGCTGGTGGCCTTCCGGAGCCACGG
  GTTTTGGCTGAAGCGGAGCAGCTACGAGGAGCAGCCGAACGTGCGCTTCCAGCACCAAGTGCTGCTCGTG
  GCTCTCCTGGGGCCGGAGCCTGAAGCCTTCCTCGCCTGGAGCACGTTCCCCACCTTCAACCGGCTGCAGG
  GGGCTCACCTGCGCGTCCCGCTCGTGTCGTCCAGAGAAGAAGACCGGAACCAGGATGGAAAAATGGATGT
  GCTGTACTTTAAACTTGAACTTCCCCTGCAACCCACAGAGCATGTCCTCGGTGTGCAGCTAATCCTGACC
  TTCTCCTACCAACTGCACAGGATGTCAACATTCGAGATGCAGAGCATGGCCTTTCTCCAGTCCTCCTTTG
  CTGTCCCTGGGTCACAGTTATATGTGAATGGCGACCTGAGGCTGCAACAGAAGCAGCCACTGAGCTACCG
  TGGCCTGGACATCAGATACAACGTGTCCGTGATCAATGGGACAAGTCCTTTTGCCCAGGACTACGACCTC
  ACCCACATTGTTGCCGCCTATCAGGAAAGAAATGTTACCACTGTCCTCAGTGACCCCAACCCGATCTGGC
  TGGTGGGCAGAGCCGCTGAAGCTCCGTTCGTGATCCATGCTGTCATCCGCTATCCCACGGAAGTCATTTC
  TTACCAGCCAGGGTTCTGGGAGATGATCAAGTTCGCCTGGATCCAGTATGTCAGCATCCTGCTGATCTTC
  CTCTGGGTGTTTGAAAGAATCAAAATCTTCGTGTTTCAGAACCAAGTAGTGACCTCTATCCCTGTAGCCG
  TGCCCCAGGGAGAGATACGGAAAGAGCACTTATCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001033321
• Insert Size: 948 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001033321.1, NP_001028493.1
• RefSeq Size: 2887 bp
• RefSeq ORF: 948 bp
• Locus ID: 234740
• UniProt ID: Q3U284
• Cytogenetics: 8 E1
• Gene Summary: Transmembrane component of the tectonic-like complex, a complex localized at the transition zone of primary cilia and acting as a barrier that prevents diffusion of transmembrane proteins between the cilia and plasma membranes. Required for ciliogenesis and sonic hedgehog/SHH signaling.[UniProtKB/Swiss-Prot Function]