Pcid2 (NM_178708) Mouse Untagged Clone

CAT#: MC212980

Pcid2 (untagged) - Mouse PCI domain containing 2 (Pcid2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pcid2
• Synonyms: A730042J05Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC212980 representing NM_178708
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCACATCACCATTAACCAGTACCTACAGCAGGTATATGAAGCCATCGACACCAGAGATGGAGCAT
  CCTGTGCAGAGCTGGTATCCTTTAAGCACCCTCATGTTGCGAACCCACGGCTCCAGATGGCCTCTCCAGA
  AGAGAAATGCCAGCAAGTTCTGGAGCCCCCATATGATGAAATGTTTGCAGCTCATTTAAGGTGTACCTAT
  GCTGTGGGGAATCACGACTTCATAGAAGCGTACAAGTGCCAGACGGTGATAGTCCAATCATTCCTCCGGG
  CATTCCAGGCTCACAAAGAGGAAAACTGGGCTCTGCCTGTCATGTATGCAGTAGCACTTGACCTTCGGAT
  ATTTGCCAATAATGCAGACCAGCAGTTGGTAAAGAAAGGGAAGAGCAAAGTTGGAGACATGCTGGAAAAA
  GCTGCCGAGCTGCTGATGAGCTGCTTCCGAGTCTGTGCCAGTGACACCCGTGCCGGCATAGAAGACTCTA
  AGAAGTGGGGGATGCTGTTCCTGGTGAACCAGCTATTTAAAATCTACTTTAAGATCAACAAACTCCATTT
  GTGTAAACCTCTTATCAGAGCCATCGACAGCTCGAATCTGAAAGATGACTATAGCACAGCGCAGAGGATC
  ACGTACAAGTACTACGTGGGGCGCAAGGCCATGTTTGACAGTGACTTCAAGCAAGCGGAGGAGTACCTGT
  CGTTTGCCTTTGAACACTGCCATCGCTCCAGCCAGAAGAACAAGAGGATGATTCTGATCTACCTGCTGCC
  AGTGAAGATGCTGCTGGGTCACATGCCGACCATCGAGCTCCTGAGGAAATACCACCTGATGCAGTTTTCA
  GAAGTGACCAAAGCCGTCAGTGAGGGCAACCTGCTGCTGCTCAATGAGGCGCTGGCAAAGCACGAGACGT
  TCTTCATCCGCTGTGGCATCTTCCTCATCCTGGAGAAGCTGAAGATCATCACGTACAGGAACCTCTTCAA
  GAAAGTGTATTTGTTACTCAAGACACATCAGCTGTCCCTAGACGCCTTTCTGGTCGCTTTGAAGTTCATG
  CACGTGGAAGACGTGGACATTGACGAGGTCCAGTGCATTCTGGCCAACTTGATATACATGGGTCACATTA
  AAGGCTACATCTCCCATCAGCATCAGAAACTGGTCGTCAGCAAGCAGAACCCTTTCCCTCCGCTGTCCAC
  AGTGTGCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_178708
• Insert Size: 1200 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_178708.3, NP_848823.2
• RefSeq Size: 2408 bp
• RefSeq ORF: 1200 bp
• Locus ID: 234069
• UniProt ID: Q8BFV2
• Cytogenetics: 8 A1.1
• Gene Summary: Required for B-cell survival through the regulation of the expression of cell-cycle checkpoint MAD2L1 protein during B cell differentiation (PubMed:20870947). As a component of the TREX-2 complex, involved in the export of mRNAs to the cytoplasm through the nuclear pores (By similarity). Binds and stabilizes BRCA2 and is thus involved in the control of R-loop-associated DNA damage and transcription-associated genomic instability. R-loop accumulation does not increase in PCID2-depleted cells (By similarity).[UniProtKB/Swiss-Prot Function]