Skint11 (NM_001166027) Mouse Untagged Clone

CAT#: MC212886

Skint11 (untagged) - Mouse selection and upkeep of intraepithelial T cells 11 (Skint11), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Skint11
• Synonyms: A630098G03Rik; Gm569
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC212886 representing NM_001166027
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAACCTTCAGCTTCCTGTTTACCTGGATTCTTCATGGTCTGCATTCTCCTAAAGATCACAGTACTCA
  CCCAAGTAATGAGCTTGGATATACAGATAAACACCCAGATTCCAGATACAGAAGAAGGGGTACTGGTAGA
  GTGTACTGCAGAAAGTTTGTTCCCACCTGCTGAGATGACATGGAGAGACAGCAAGGGGAATATAATTCCA
  CCCTCAAGCACATTTGACTCTCAGGATAGAGCTGGGTTATTGTGTTTGAAGAGCACCATTCTTTTGAAAA
  ACAGAACAGAAGGCCCTATTACTTGCTCCATTTACAATAAAACTACTAATCAGGAGAAAAGGAGAAGTAT
  TATCCTATCAGATGTCCTATTCAGACCACAGTATATGTCATTGATGTCAAACAATCTTTTATATCTTGGA
  ATATATTTAATCTTTATACTTTTTCTTAATTTTCTCAAAGGAATACTTTTTTGTCTCACAAAAAGACTTG
  TTCATTTCAGAAAACGAATGATTAAAATCAAGAAAGTTTGGAGTAATAAAACAAGAGCTTGCTGTCCACT
  TATCTGGGAGTTCCTAGAGATAGTTTTGTTTATTGCCTTCCTCCCTCTCTACTTGATGTTCAGGATTAGA
  GTTTTTACTTTGGATGAGGCACACATATTGTATAATAATTGGCTGTGGAAAGTATGTAAAACTTTGATTG
  CAATGATGATACTTTTTACTGTGCTCATTTTGTTTCTACTTTGGACCCTGAACCGTTATGGTAAGATGCC
  CTGCTTATCTTCCATGAATATAGATGTTTCTACACATGACGCGGAGCAAAACAGTTCTAAATCTGCTAAG
  TTCCAAGAAAATTATGATGTAGCAGGCCAAATGATCCTAGAAACTTATGAAGAAACAATATTTTGTCAGC
  ATCAAGAAAGTTGTGAAGAGTATAATTATGACCCTCTTCTGCTCTCAAGTTTAGATGCCCTAGGAACTTG
  TGAAGATGAAAAATTTAGTCAGCATCAAGAAAGTTTTGAAGAGGATGAGGATCTTCAATCTTTTTCAGAC
  TTTAAGATTGAATTGTACTCGAAACTTGGAAACTTGACACATTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001166027
• Insert Size: 1095 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001166027.1, NP_001159499.1
• RefSeq Size: 1393 bp
• RefSeq ORF: 1095 bp
• Locus ID: 230623
• UniProt ID: A7XV14
• Cytogenetics: 4 D1
• Gene Summary: May act by engaging a cell surface molecule on immature T-cells in the embryonic thymus.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).