Kyat3 (NM_173763) Mouse Untagged Clone

CAT#: MC212873

Ccbl2 (untagged) - Mouse cysteine conjugate-beta lyase 2 (Ccbl2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Kyat3
• Synonyms: Ccbl2; Kat3; KATIII
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC212873 representing NM_173763
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTTGAAATTCAAAAACGCCAAACGAATCGAAGGCCTGGACAGCAATGTGTGGGTTGAATTTACTA
  AGTTGGCTGCGGATCCTTCTGTGGTGAACCTTGGACAAGGCTTTCCAGATATATCCCCTCCTTCATACGT
  AAAAGAAGAGTTATCAAAGGCTGCATTTATTGATAACATGAATCAATACACAAGAGGCTTTGGTCATCCA
  GCACTTGTGAAAGCTCTGTCCTGCTTATATGGAAAGATTTATCAACGTCAAATTGATCCAAACGAAGAAA
  TCCTTGTGGCCGTGGGGGCATATGGATCTCTCTTTAACTCCATCCAAGGATTGGTGGACCCAGGAGATGA
  AGTGATAATTATGGTGCCTTTTTACGACTGTTATGAGCCCATGGTGAGAATGGCCGGAGCAGTGCCTGTG
  TTTATTCCCCTGAGATCTAAACCTACTGACGGGATGAAGTGGACTAGCTCTGACTGGACATTCGATCCTC
  GAGAACTGGAAAGTAAATTCAGTTCCAAAACGAAAGCCATAATATTAAATACTCCACACAACCCCCTCGG
  CAAGGTGTATACCAGACAGGAGCTGCAAGTCATTGCTGACCTTTGCGTCAAGCACGACACTCTGTGCATC
  AGCGATGAGGTTTATGAATGGCTTGTCTATACTGGACATACGCACGTAAAAATAGCCACTCTTCCAGGTA
  TGTGGGAGAGAACAATAACAATAGGAAGTGCTGGCAAGACATTCAGTGTGACTGGCTGGAAGCTCGGCTG
  GAGCATTGGCCCTGCTCACCTGATAAAGCATTTACAGACCGTTCAACAGAACAGTTTTTACACGTGTGCG
  ACTCCTTTACAGGCAGCCTTGGCCGAGGCGTTTTGGATCGATATCAAGCGCATGGATGACCCTGAGTGTT
  ACTTTAATTCTCTGCCAAAGGAATTAGAAGTAAAGAGAGATCGGATGGTCCGTTTACTTAACAGCGTTGG
  CCTGAAACCCATTGTTCCTGACGGGGGTTACTTCATCATTGCTGATGTGTCTTCATTAGGTGCTGACCTC
  TCGGACATGAACAGCGATGAGCCTTATGACTATAAGTTTGTGAAGTGGATGACGAAACATAAGAAACTGA
  CAGCCATTCCTGTTTCTGCCTTCTGCGACTCCAAGTCTAAACCACACTTTGAGAAGCTGGTGCGGTTTTG
  CTTTATTAAAAAAGACAGCACACTGGATGCTGCCGAAGAAATCTTCAGGGCCTGGAACAGCCAGAAGTCT
  TGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_173763
• Insert Size: 1263 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_173763.4, NP_776124.1
• RefSeq Size: 2279 bp
• RefSeq ORF: 1263 bp
• Locus ID: 229905
• UniProt ID: Q71RI9
• Cytogenetics: 3 H1
• Gene Summary: Catalyzes the irreversible transamination of the L-tryptophan metabolite L-kynurenine to form kynurenic acid (KA). May catalyze the beta-elimination of S-conjugates and Se-conjugates of L-(seleno)cysteine, resulting in the cleavage of the C-S or C-Se bond (By similarity). Has transaminase activity towards L-kynurenine, tryptophan, phenylalanine, serine, cysteine, methionine, histidine, glutamine and asparagine with glyoxylate as an amino group acceptor (in vitro). Has lower activity with 2-oxoglutarate as amino group acceptor (in vitro).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR and uses an alternate splice site in a 5' exon resulting in the use of a downstream start codon compared to variant 1. The encoded isoform (2) has a shorter N-terminus compared to isoform 1.