Spout1 (NM_172660) Mouse Untagged Clone

CAT#: MC212809

D2Wsu81e (untagged) - Mouse DNA segment, Chr 2, Wayne State University 81, expressed (D2Wsu81e), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Spout1
• Synonyms: 9930028P05; AL033358; CENP-32; D2Wsu81e
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC212809 representing NM_172660
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAGCGCCCGAGGAAGCGTCCGTGTGGTCCGGGTGAACATGGCCAAAGGGTTGAATGGCGAAAAT
  GGAAACAGCAGAAAAAAGAGGAGAAAAAGAAGTGGAAGGACCTCAAGATAATGAAGAAACTGGAGCGGCA
  GCGTGCCCAAGAGGAAGAGGCTAAACGCCAGGAAGAGGAGGAAGAGGCGGCGGCCCAGAGGAGCAACCAA
  GGACGGCCTTACACTCTGAGTGTGGCCCTGCCAGGCTCCATCCTGGACAATGCCCAGTCACCTGAGCTCC
  GCACCTACCTGGCTGGCCAGATCGCCAGAGCCTGCACCATCTTCTGTGTGGATGAGATTGTGGTGTTCGA
  TGAGGAGGGCCAAGATACCAAGAGTGTGGAAGGAGAATTCAGGGGAGTTGGCAAGAAAGGGCAGGCATGT
  GTCCAGCTGGCCCGCATCCTACAGTACCTGGAGTGTCCACAGTACCTGAGAAAGGCGTTCTTCCCCAAGC
  ACCAAGATCTGCAGTTTGCAGGAATCCTCAACCCCTTGGACAGCCCTCACCACATGCGTCAGGACGAAGA
  GTCTGAGTTTCGAGAAGGCGTCGTGGTGGACCGGCCCACCAAGGCAGGCCATGGCTCTTTGGTCAACTGT
  GGAATGAAGAAGGAGGTGAAGATTGACAAGAAACTGGACCCTGGACTTCGGGTGACCGTGCGACTGAACC
  AGCAGCAGCTCCCAGAATGCAAGACATACAAAGGAACAGTTGTGTCATCACAGGACCCCCGCACCAAAGC
  TGGTCTCTACTGGGGCTACACTGTCCGGCTGGCCTCTTGCCTCAGCGCTGTGTTTGCTGAGGCTCCCTTC
  CAGGATGGGTACGACCTGACCATTGGGACATCAGAACGTGGCTCCGACGTGGCCTCTGCCCAGCTGCCCA
  GCTTCAGGCATGCCCTTGTGGTGTTTGGAGGCCTCCAAGGGCTAGAAGCTGCAGTGGATGCTGACCCGAA
  CCTTGAAGTGGCTGACCCCAGTGTCCTCTTCGACTTCTATGTCAACACATGCCTTAGCCAGGGCAGCCGT
  ACCATCCGCACCGAGGAGGCCATACTCATCTCGCTGGCTGCTCTGCAGCCTGGTCTCACCCAGGTGGGAT
  CGAGGCCCGCCTCCCCGCTTTCTGGACCCAGGATGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_172660
• Insert Size: 1158 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172660.4, NP_766248.3
• RefSeq Size: 1838 bp
• RefSeq ORF: 1158 bp
• Locus ID: 227695
• UniProt ID: Q3UHX9
• Cytogenetics: 2 21.29 cM
• Gene Summary: Required for association of the centrosomes with the poles of the bipolar mitotic spindle during metaphase. Also involved in chromosome alignment. May promote centrosome maturation probably by recruiting A-kinase anchor protein AKAP9 to centrosomes in early mitosis. Binds specifically to miRNA MIR145 hairpin, regulates MIR145 expression at a postranscriptional level (By similarity).[UniProtKB/Swiss-Prot Function]