Sohlh1 (NM_001001714) Mouse Untagged Clone

CAT#: MC212805

Sohlh1 (untagged) - Mouse spermatogenesis and oogenesis specific basic helix-loop-helix 1 (Sohlh1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Sohlh1
• Synonyms: Gm110; Nohlh
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC212805 representing NM_001001714
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTCCGGTGGCCACGAGCGGGCCAATGAGGATTACAGAGTCTCTGGCATTACGGGATGCAGCAAGA
  CTCCTCAGCCTGAGACTCAGGACAGCTTGCAGACCTCATCACAAAGCTCAGCTCTCTGCACAGCTCCTGT
  GGCTGCTGCAAACTTGGGCCCCAGTCTTCGGAGAAACGTGGTCAGCGAGAGAGAACGCAGGAGGCGGATC
  TCGTTGAGCTGTGAGCACTTGCGGGCTCTACTGCCTCAGTTTGATGGCCGACGGGAGGACATGGCATCTG
  TCCTGGAGATGTCTGTGTACTTCCTCCAGCTTGCCCACAGCATGGACCCTAGCTGGGAGCAACTCTCTGT
  TCCTCAGCCTCCCCAGGAGATGTGGCACATGTGGCAGGGTGATGTTCTGCAGGTAACCCTGGCGAATCAG
  ATTGCAGACAGCAAGCCAGACTCCGGTATAGCCAAACCATCTGCTGTGTCTCGGGTACAGGATCCCCCAT
  GCTTTGGGATGCTGGATACAGACCAGAGCCAGGCTACTGAGAGAGAGTCAGAGCTGCTGGAGAGACCTTC
  CTCCTGCCCTGGTCATCGCCAGAGCGCGTTGTCATTCAGTGAGCCAGAGTCTTCCAGCTTGGGTCCTGGG
  CTCCCACCCTGGATCCCTCACTCATGGCAGCCAGCCACTCCCGAGGCAAGTGACATTGTTCCTGGTGGGT
  CACACCAGGTGGCATCCCTGGCCGGGGACCCTGAATCTTCCGGCATGCTGGCTGAGGAGGCCAACTTGGT
  CTTGGCATCTGTGCCTGATGCCAGGTACACCACAGGGGCAGGGTCCGATGTGGTGGATGGAGCACCCTTT
  CTGATGACCACCAATCCTGACTGGTGGTTGGGGTCGGTGGAGGGCAGAGGAGGCCCAGCCCTTGCCAGGA
  GCAGCCCAGTGGATGGGGCAGAGCCAAGCTTCATCGGAGACCCTGAGCTTTGCTCCCAGGAGCTCCAGGC
  TGGTCCTGGAGAGCTGTGGGGTTTGGATTTTGGCAGCCCTGGCCTGGCCCTGAAGGATGAAGCGGACAGC
  ATCTTCCCTGACTTTTTCCCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001001714
• Insert Size: 1074 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001001714.1, NP_001001714.1
• RefSeq Size: 1314 bp
• RefSeq ORF: 1074 bp
• Locus ID: 227631
• UniProt ID: Q6IUP1
• Cytogenetics: 2 A3
• Gene Summary: Transcription regulator of both male and female germline differentiation. Suppresses genes involved in spermatogonial stem cells maintenance, and induces genes important for spermatogonial differentiation (PubMed:22056784). Coordinates oocyte differentiation without affecting meiosis I (PubMed:28504655).[UniProtKB/Swiss-Prot Function]