A3galt2 (NM_001009819) Mouse Untagged Clone

CAT#: MC212623

A3galt2 (untagged) - Mouse alpha 1,3-galactosyltransferase 2 (isoglobotriaosylceramide synthase) (A3galt2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: A3galt2
• Synonyms: Gm433; iGb3; iGb3S
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC212623 representing NM_001009819
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCTGGGGACAGAGTTGGGAGTGAGCTGGCCAGGGTCACATGGAAGTTGCCGAGAACAAGAAGGAC
  AGAGACAAAGAGGCCCAGGGAAGCCAACCTGGGGACTTTCACGGGCCAAGAAGAGACTCCTGTGGCGGTT
  CTTCCTGTCTGCATTTGGTTTCTTAGGCCTGTACCATTACAGGTTCATTATTATCAGGCTCATAGAAGGC
  TCCATCCCCATGGGCACCTGCCCTACAGCCATAATGCCTCTGCCGAGGGACAATTTCACAGGAGTACTGC
  ACCACTGGGCCCGGCCTGAAGTCCTGACCTGTACCTCTTGGGGAGCACCAATTATTTGGGATGGCACTTT
  CGACCCTCATGTAGCCCAGCAAGAGGCGAGACGGCGGAACCTCACCATCGGGCTGACTGTGTTTGCTGTA
  GGCAGGTACCTGGAGAAGTACCTGGAACACTTCCTGGTATCGGCAGAGCAGCACTTCATGGTCGGCCAGA
  ACGTGGTGTACTATGTGTTTACGGATCGCCCGGAAGCAGTGCCCTATGTGGCTCTAGGCCAGGGTCGCCT
  GCTGCGGGCAAAACCCGTGCAGCGAGAGAGGCGCTGGCAGGACGTGTCCATGGCACGCATGCCCACGCTA
  CACGAGGCTCTGGGAGGGCAGCTGGGCCAAGAAGCTGACTTTGTGTTCTGCCTGGACGTGGACCAGTACT
  TCACCGGTAACTTCGGGCCTGAGGTGCTGGCAGATTTGGTGGCACAGCTGCACGCCTGGCACTACCGCTG
  GCCGCGGTGGCTGCTGCCCTACGAGAGGGACAAGCGATCGGCTGCTGCGCTGTCGTTAAGCGAAGGCGAT
  TTCTACTACCACGCTGCGGTGTTTGGCGGCAGTGTGGCTGCACTGCTCAAGCTGACGGCCCACTGTGCGA
  CTGGCCAACAGCTGGACCATAAGCGCGGCATTGAGGCACTCTGGCACGACGAAAGCCACCTTAACAAGTT
  CTTCTGGCTGAACAAGCCCACCAAGCTGCTGTCGCCTGAGTTCTGCTGGGCAGAGGAAATTATCTGGAGG
  AGAGAGATCCATCACCCACGCCTGCTCTGGGCACCCAAGGAATATACGCTGGTGCGAAACTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001009819
• Insert Size: 1113 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001009819.2, NP_001009819.1
• RefSeq Size: 3390 bp
• RefSeq ORF: 1113 bp
• Locus ID: 215493
• UniProt ID: Q3V1N9
• Cytogenetics: 4 D2.2
• Gene Summary: Synthesizes the galactose-alpha(1,3)-galactose group on the glycosphingolipid isoglobotrihexosylceramide or isogloboside 3 (iGb3) by catalyzing the transfer of galactose from UDP-Galactose to its acceptor molecule Gal-beta-1,4-Glc-ceramide. Can also catalyze the addition of galactose to iGb3 itself to form polygalactose structures. Synthesis of iGb3 is the initial step in the formation of the isoglobo-series glycolipid pathway and is the precursor to isogloboside 4 (iGb4) and isoForssman glycolipids. Can glycosylate only lipids and not proteins and is solely responsible for initiating the synthesis of isoglobo-series glycosphingolipids.[UniProtKB/Swiss-Prot Function]