Chpt1 (NM_144807) Mouse Untagged Clone

CAT#: MC212576

Chpt1 (untagged) - Mouse choline phosphotransferase 1 (Chpt1), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Chpt1
• Synonyms: MGC28885
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC212576 representing NM_144807
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGGGCGCCGGGGCCAGGCCGGCGCCGCGCTGGGTAAAGGCGCTAGGTGAGCCGCTCAGTGCCG
  CGCAGCTGCGGCGGCTGGAGGAGCACCGCTACACCGCGGTGGGAGAGTCGCTGTTCGAGCCGCCGCTGCA
  GCTTTACTGGACCTGGCTGCTCCAATGGATCCCGCTCTGGATGGCCCCCAACACCATCACCCTCATCGGC
  CTCGCCATCAACCTGGTCACCACACTAGTGCTCATCTTCTACTGCCCTACAGTCACGGAGGAGGCACCAT
  ACTGGACATACCTTTTATGTGCCCTGGGACTCTTTATCTACCAGTCACTGGATGCCATTGATGGGAAACA
  AGCCAGAAGGACAAACTCTTGCTCTCCCTTAGGGGAACTATTTGATCATGGTTGTGACTCTCTTTCCACA
  GTATTTATGGCCATCGGCGCTTCCATTGCTGTTCGCCTAGGAACACATCCTGACTGGTTGTTTTTCTGCT
  CTTTCGTTGGGATGTTCATGTTTTACTGTGCTCATTGGCAGACTTACGTCTCAGGAGTGTTAAGATTTGG
  AAGGGTGGATGTCACTGAGATCCAGGTAGCTTTAGTGATCGTCTTCATGTTGTCAACATTTGGAGGAGCA
  ACAATGTGGGACTATACGATACCTATTCTAGAAATAAAACTGAAGATTGTTCCGGTTCTTGGAGTTGTAG
  GTGGATTAATATTTTCCTGTTCAAATTATTTCCATGTGATCCTCCATGGTGGTGTTGGCAAGAATGGGTC
  TACTATTGCAGGCACCAGTGTCTTGTCACCTGGACTCCACATAGGATTAATTATTATATTGGCAATAATG
  ATCTATAAGAAGTCTGCAACAAATATGTTTGAAAAACATCCTTGCCTTTATACTTTAATGTTTGGATGTG
  TCTTTGCTAAAGTTGCACAAAAATTGGTGATAGCTCACATGACGAAAAGTGAACTATATCTTCAAGACAC
  TGTCTTTATTGGGCCAGGTCTTTTATTTTTAGACCAATACTTTAATAATTTTATAGATGAATATGTTGTT
  CTGTGGATAGCAATGGTCATTTCTTCATTTGATATGATGATATACTTTACTTCTTTGTGCCTGCAAATTT
  CAAGACACCTTCATCTAAACATCTTCAAGACTTCATGTCAACAAGCACCGGAACAGGTTTACAAGCATAT
  TGACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_144807
• Insert Size: 1197 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_144807.3, NP_659056.3
• RefSeq Size: 3239 bp
• RefSeq ORF: 1197 bp
• Locus ID: 212862
• UniProt ID: Q8C025
• Cytogenetics: 10 C1
• Gene Summary: Catalyzes phosphatidylcholine biosynthesis from CDP-choline. It thereby plays a central role in the formation and maintenance of vesicular membranes (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) contains a different segment for its 3' coding region and 3' UTR versus variant 1. The resulting protein (isoform 2) has a shorter and distinct C-terminus when it is compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.