Cldn16 (NM_053241) Mouse Untagged Clone

CAT#: MC212266

Cldn16 (untagged) - Mouse claudin 16 (Cldn16), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cldn16
• Synonyms: claudi; claudin-16; PC; PCLN1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC212266 representing NM_053241
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGGATCTTCTTCAGTACGCTGCCTGCTTCTTGGCCATATTCTCCACTGGGTTTTTGATCGTGGCCA
  CCTGGACAGACTGTTGGATGGTGAACGCTGATGACTCCCTGGAGGTGAGCACTAAATGCAGAGGCCTGTG
  GTGGGAGTGTGTAACAAACGCTTTTGATGGGATTCGAACCTGCGATGAGTACGACTCCATATATGCAGAA
  CATCCCTTGAAGCTGGTGGTAACTCGAGCACTGATGATCACAGCTGACATTTTAGCTGGCTTTGGATTCA
  TCACCCTGCTCCTTGGTCTGGACTGTGTGAAGTTCCTACCTGATGACCCACAAATTAAAGTCCGCCTTTG
  CTTTGTTGCAGGGACCACATTACTCATTGCAGGTACCCCAGGAATCATCGGTTCTGTGTGGTATGCTGTG
  GATGTTTACGTCGAACGCTCCTCTCTCGTTTTACACAATATATTTCTTGGGATCCAATATAAATTTGGTT
  GGTCCTGCTGGCTTGGAATGGCTGGGTCTTTGGGTTGCTTTTTGGCAGGAGCTCTCCTCACCTGCTGTTT
  GTACCTCTTCAAAGATGTTGGGCCTGAGAGGAACTACCCTTATGCCATGAGGAAGCCCTATTCAACTGCA
  GGTGTGTCCATGGCCAAGTCCTACAAGGCCCCTCGGACAGAGACAGCCAAAATGTATGCTGTAGACACCA
  GAGTATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_053241
• Insert Size: 708 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_053241.5, NP_444471.1
• RefSeq Size: 1173 bp
• RefSeq ORF: 708 bp
• Locus ID: 114141
• UniProt ID: Q925N4
• Cytogenetics: 16 B2
• Gene Summary: This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. The protein encoded by this gene is critical for renal paracellular epithelial transport of Ca(2+) and Mg(2+) in the thick ascending loop of Henle. The gene deficiency leads to specific alterations in renal Ca(2+) and Mg(2+) balance and also to disturbances in Na(+) handling. The interaction of this gene and the Cldn 19 gene is required for their assembly into tight junctions and for renal Mg(2+) reabsorption. This gene and the Cldn1 gene are clustered on chromosome 16. [provided by RefSeq, Aug 2010]