Lmx1a (NM_033652) Mouse Untagged Clone

CAT#: MC212230

Lmx1a (untagged) - Mouse LIM homeobox transcription factor 1 alpha (Lmx1a), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Lmx1a
• Synonyms: dr; dreher; Lmx1.1; sst
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC212230 representing NM_033652
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTGGACGGCCTGAAGATGGAGGAGAACTTTCAAAGTGCGATTGAGACCTCGGCATCTTTCTCCTCTT
  TGCTGGGCAGAGCGGTGAGCCCCAAGTCTGTCTGCGAGGGCTGTCAGCGGGTCATCTCGGACAGGTTTCT
  GCTGCGGCTCAACGACAGCTTCTGGCACGAGCAATGCGTGCAGTGTGCCTCCTGCAAAGAGCCCCTGGAG
  ACCACCTGCTTCTACCGGGACAAGAAGCTCTACTGCAAGTACCACTACGAGAAACTGTTTGCTGTCAAAT
  GTGGGGGCTGCTTCGAGGCCATTGCGCCCAATGAGTTTGTCATGCGTGCCCAGAAGAGCGTATACCACCT
  GAGCTGCTTCTGCTGCTGCGTCTGTGAGCGACAGCTGCAGAAGGGTGACGAGTTTGTCCTGAAGGAGGGC
  CAGCTGCTCTGCAAAGGGGACTATGAGAAAGAACGGGAGCTGCTGAGCCTGGTGAGCCCTGCGGCCTCAG
  ACTCAGGCAAAAGCGATGATGAGGAGAGCCTTTGCAAGTCAGCCCATGGGGCAGGAAAAGGAGCATCAGA
  GGACGGCAAGGACCATAAGCGACCCAAACGTCCCAGAACCATCCTGACCACTCAGCAGAGGAGAGCATTC
  AAGGCCTCGTTTGAAGTATCCTCCAAGCCCTGCAGAAAGGTGAGGGAGACTCTGGCTGCGGAGACAGGGC
  TGAGTGTCCGTGTGGTTCAGGTGTGGTTCCAGAACCAGCGAGCCAAGATGAAGAAGCTGGCCCGGCGACA
  GCAGCAACAGCAACAGGACCAACAGAACACCCAGAGGCTGACTTCTGCTCAGACAAATGGTAGTGGGAAT
  GCGGGCATGGAAGGGATCATGAACCCCTATACAACGTTGCCCACCCCACAGCAGCTGCTGGCCATTGAAC
  AGAGCGTCTACAACTCTGATCCCTTCCGACAGGGTCTCACCCCACCCCAGATGCCTGGAGATCACATGCA
  CCCCTATGGTGCTGAACCTCTTTTCCATGACTTGGATAGTGATGACACATCTCTCAGTAACCTGGGAGAC
  TGCTTCCTGGCAACCTCAGAAGCTGGGCCCCTGCAGTCCAGAGTGGGAAACCCCATTGACCATCTGTACT
  CCATGCAGAATTCCTATTTCACCTCTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_033652
• Insert Size: 1149 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_033652.5, NP_387501.1
• RefSeq Size: 3338 bp
• RefSeq ORF: 1149 bp
• Locus ID: 110648
• UniProt ID: Q9JKU8
• Cytogenetics: 1 75.08 cM
• Gene Summary: Acts as a transcriptional activator by binding to an A/T-rich sequence, the FLAT element, in the insulin gene promoter. Required for development of the roof plate and, in turn, for specification of dorsal cell fates in the CNS and developing vertebrae.[UniProtKB/Swiss-Prot Function]