Gtf2a1 (NM_175335) Mouse Untagged Clone

CAT#: MC211964

Gtf2a1 (untagged) - Mouse general transcription factor II A, 1 (Gtf2a1), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Gtf2a1
• Synonyms: 6330549H03Rik; AA536742; AA959775; AW060250; Tfiia1; TfIIAa/b
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC211964 representing NM_175335
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAACTAAAAACTTTGTGGGAGAATAAACTAATGCAGTCTAGGGCAGTAGATGGATTTCATTCAGAAG
  AACAGCAGCTTTTGTTGCAAGTTCAGCAGCAGCATCAACCCCAGCAGCAGCAGCATCACCACCACCACCA
  CCAGCACCAGCAAGCGCAACCTCAGCAAACGGTACCTCAGCAAGCACAGACCCAGCAGGTCCTCATTCCT
  GCATCCCAGCAAGCTACAGCACCACAAGTTATTGTTCCTGATTCTAAGTTGCTACAACATATGAATGCAT
  CAAGTATTACGAGTGCTGCTGCTACAGCTGCTACCTTGGCACTGCCTGCAGGAGTGACTCCGGTTCAACA
  GCTACTAACAAATTCAGGCCAGCTGCTTCAGGTGGTCAGAGCAGCCAATGGTGCCCAGTATATCTTGCAG
  CCTCAGCAGTCAGTTGTTCTACAACAACAAGTTATACCACAAATGCAGCCTGGTGGAGTCCAAGCTCCTG
  TTATCCAGCAGGTCTTAGCCCCACTTCCTGGAGGGATTTCACCACAGACAGGCGTCATCATCCAGCCACA
  GCAAATCTTATTTACAGGAAATAAGACTCAGGTCATTCCTACAACAGTGGCAGCACCCGCCCCGGCACAA
  GCGCCGATGCCTGCAGCCGGACAGCAGCAGCCACAGGCCCAGCCTGCCCAACAGCAAGCTCCGTTGGTGC
  TGCAAGTTGATGGAACAGGGGATACATCTTCTGAGGAAGATGAAGATGAAGAAGAAGACTATGATGATGA
  TGAGGAAGAAGATAAAGAGAAAGATGGAGCTGAAGATGGGCAGGTGGAAGAAGAGCCGCTCAACAGCGAG
  GATGACGTCAGTGACGAGGAAGGGCAGGAACTCTTTGACACTGAGAATGTTGTCGTCTGCCAGTATGATA
  AGATACACAGAAGTAAAAATAAATGGAAATTTCATCTCAAGGATGGCATTATGAATCTTAACGGAAGAGA
  TTATATATTTTCCAAAGCCATTGGAGATGCAGAGTGGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_175335
• Insert Size: 1020 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_175335.3, NP_780544.1
• RefSeq Size: 5691 bp
• RefSeq ORF: 1020 bp
• Locus ID: 83602
• UniProt ID: Q99PM3
• Cytogenetics: 12
• Gene Summary: TFIIA is a component of the transcription machinery of RNA polymerase II and plays an important role in transcriptional activation. TFIIA in a complex with TBP mediates transcriptional activity (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) has multiple differences in the presence and absence of exons at its 5' end, compared to variant 1. These differences produce a unique 5' UTR and cause translation initiation at a downstream start codon, compared to variant 1. The encoded protein (isoform 2) is shorter than isoform 1.