Ccdc125 (NM_001168386) Mouse Untagged Clone

CAT#: MC211705

Ccdc125 (untagged) - Mouse coiled-coil domain containing 125 (Ccdc125), transcript variant B, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ccdc125
• Synonyms: 5830436D01Rik; AL023012
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC211705 representing NM_001168386
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCAAAGTGCCGAGGTCATCGAGCGAGGCAGAGGACATCTGGGAAACAGAGGATGACATGACCGAAG
  GTGACCTAGGCTATGGCCTCGGAAGAAAACCCGGTGGTATTTATGAAGTTCCGTGTTCGATTACATCTAA
  GAAAAGGTCAGATGGAAAGAACTCAAGCCCTCCTCCGTTTCCAAGAAAGGGAGAAGAAAGAAGCGAAACC
  AGTTTCCAGTATTCCAGGAGGAAGGGCTTTCAAGATACAAGCGCTGAGGGATACCGAGCATCCAGACTGA
  GCAGCACAGATTCTAACTCAGAATTGTCGGATGAGCAGTTAAGGCGACGTCTTCATGAAGCCTTAGAGGA
  CGTAGAGATTTTAAAAACGGAACTTGAAGCGTCTCAAAGACAACTTGAAGGTAAAGAGGAAGCATTGAAA
  ATCCTCCAAAGCATGGAAATAAATGCCTTGCAGTGGGAAATGGAATTTGATCAGGATAGATTTAAAAATA
  TAGAAGAATCTTGGATCCAGAAATGTGACAGGCTAAACTGTGACAATGCAGTCCTCAGAGAGAATCTGAA
  GTTGAGAACAGAGGAAATAAAGATGCTAAAGTCTAAGAATGCTGTTTTGAATCAGCGGTACTTGGAGGCC
  CTCGCCATGCTTGATATCAAGGAGCAGAAGATGGGTCAGGAGGAGAGTGGCTTTACAGATGTATCAGGTC
  TCGAGCTTGCAGTCCTTGGAGCCTGCCTGTGTCATGGTCCTGGAGGGAGCCCCTGTTCTTGTGCCAAAAT
  GGCAGCATCCACTCGGAAACTGGTTCTTCAGCTCAGACATGAGTTGGAAACTCTGCAGAAGAGTAAGGAA
  GAGGCGCACATAACGGCAGATGCATTCAGGATTGCTTTTGAGCAACAGTTAATGAGGAAAAATGAGCAGG
  CACTGAGACTGGCTGGAGGGGACCTGTGTAAAAGAGCGGCAACGTGGATCAACAGACAACACCAGGCAGA
  CGATGTTGAATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001168386
• Insert Size: 993 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001168386.2, NP_001161858.1
• RefSeq Size: 2496 bp
• RefSeq ORF: 993 bp
• Locus ID: 76041
• UniProt ID: Q5U465
• Cytogenetics: 13 D1
• Gene Summary: May be involved in the regulation of cell migration.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (B) lacks an in-frame exon in the 5' coding region and an exon in the 3' coding region, which results in a frameshift, compared to variant A. The resulting protein (isoform B) is shorter and has a distinct C-terminus, compared to isoform A. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.