Sh2d4a (NM_028182) Mouse Untagged Clone

CAT#: MC211311

Sh2d4a (untagged) - Mouse SH2 domain containing 4A (Sh2d4a), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Sh2d4a
• Synonyms: 2210402M20Rik; SH2A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC211311 representing NM_028182
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGAGGCAGATACTGTCAGATATGTTCATAGACCCTGACCTGCTGGCAGAGCTCAGCGAAGAGCAGA
  AACAGATCTTGTTCTACAAGATGAGAGAGGAACAGATCCGACGATGGAAAGAAAGAGAAGCGGCCATGGA
  AAGAAAGGAGTCCTTGCCAGTGAAATCCAGGCCAAAAAAAGAGAATGGCAAGTCTGTCCATTGGAAGCTG
  GGCGCCGATAAGCAGGTGTGGGTTTGGGTAATGGGCGAGCACCATCTGGACAAACCCTATGATGTGCTGT
  GTGATGAGATCCTTGCGGAGAGGGAGCATCTGAGAGCAGCGAAGGATTCAGAGCTCAGGAAAACTCAGTC
  TCTAGAACTCGCCAATAGCTTAAAAATAAAGTCACAGAACTGTGATCTGCAAGCAATGAAGAAGACAGAG
  CCTCAGAATGTCACCAGGAAAGCAGCTTCAGAAGAGGCATCAGGTCAAGGACCCAGAGCAATACCAACCA
  GGAAGGATGACAAAGCCCAAACTAAACCCGTCAAGGAAAAAGACCACGAGGAAATGAAGCAGACAGAGGA
  TGAGAAAACCAAGCAGATATACAAGAGCTGGAAAGAAGACTCAGAATGGCAAGCATCTCTGCGAAAATCT
  AAGGCGGCTGATGAGAAGAGACGCTCTTTAGCTAAACAAGCACGGGAAGACTACAAGAGGCTATCCCAAA
  GGGGGAGGAGTGGGGACGGACTGCAGAACCCACTGACAGGTCCACAGAAGCCCAGAAGACCTCCTCTTCC
  TCCGAAGCCCCAGTTCCTACAGCCGCTGGGAATCCCTCCAAAGTCTTTAGGGAATCAGGGGGTGATAAGG
  ACCGAGATCAGCTCCGCCCAGATGGACACCATTCGATGGTTCAAAGAGGAACAGTTGCCATTCCGTGCAG
  GTTACCAGAAAAACTCAGACACCATTGCTCCTTGGTTCCATGGGATTCTCACACTGAAGAAAGCAAATGA
  ACTTCTGAGCACAGGTGTGCCGGGAAGTTTTTTGATTCGAGTCAGTGAAAAGATCAAGGGCTATGCCCTG
  TCCTACCTGTCTGAGGAAGGCTGCAAACATTTCCTTATAGATGCATCTGCCAACTCTTACAGCTTCCTGG
  GTGTGGACCAGCTGCAGCATGCTACACTGGCAGATTTGGTGGAATATCACAAGGAGGAGCCCATAACCTC
  TCTGGGGAAGGAACTCCTTCTGTACCCCTGTGGTCAACAAGACAAGCTGCCCGACTACCTGGAGCTCTTC
  CAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_028182
• Insert Size: 1266 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_028182.1, NP_082458.1
• RefSeq Size: 2767 bp
• RefSeq ORF: 1266 bp
• Locus ID: 72281
• UniProt ID: Q9D7V1
• Cytogenetics: 8 B3.3
• Gene Summary: Inhibits estrogen-induced cell proliferation by competing with PLCG for binding to ESR1, blocking the effect of estrogen on PLCG and repressing estrogen-induced proliferation (By similarity). May play a role in T-cell development and function.[UniProtKB/Swiss-Prot Function]