Tmem25 (NM_027865) Mouse Untagged Clone

CAT#: MC211214

Tmem25 (untagged) - Mouse transmembrane protein 25 (Tmem25), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tmem25
• Synonyms: 0610039J01Rik; AI429491
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC211214 representing NM_027865
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAATTGCCTCTAAGCCAAGCTACCCTCCGGCACACACTGCTGCTCCTTCCTGCCCTCTTAAGTTCAG
  GTCAAGGGGAGCTGGCGCCGCAAATTGATGGTCAGACCTGGGCTGAGAGAGCGCTTAGGGAGAATGAGCA
  CCATGCCTTCACCTGTCGGGTTGCAGGAGGTTCTGCCACTCCCCGATTAGCCTGGTACCTGGATGGACAG
  CTACAGGAGGCCACCACCTCCCGACTGCTGAGTGTGGGTGGGGACGCCTTCTCTGGAGGAACCAGCACTT
  TCACTGTTACTGCCCAGCGCTCCCAACATGAGCTTAACTGCTCCCTGCAGGACCCAGGGAGTGGCCGGCC
  AGCCAATGCCTCTGTCATCCTCAATGTGCAATTCAAACCAGAGATTGCCCAGGTTGGAGCCAAATACCAG
  GAAGCTCAGGGCCCAGGCCTTCTGGTTGTCCTGTTTGCCCTGGTGCGGGCCAACCCACCTGCCAATGTCA
  CCTGGATTGACCAGGATGGGCCAGTGACTGTCAATGCCTCTGACTTCCTGGTGCTGGATGCTCAGAACTA
  TCCCTGGCTCACCAACCACACTGTGCAGCTGCAGCTCCGGAGCTTGGCACACAACCTCTCGGTGGTGGCC
  ACCAATGACGTGGGTGTCACTAGCGCTTCCCTTCCAGCTCCAGGGCTCCTGGCCACACGGATAGAAGTGC
  CACTGCTGGGCATCGTTGTGGCTGGAGGACTTGCCTTGGGCACTCTAGTGGGGTTCAGTACCTTGGTGGC
  CTGCCTGGTCTGCAGGAAAGAGAAGAAAACAAAAGGCCCCTCCCGGCGTCCTTCTCTGATATCTAGTGAC
  TCCAATAACCTGAAACTGAATAATGTGCGCCTGCCACGGGAGAACATGTCCCTCCCATCCAACCTTCAGC
  TCAATGACCTCACTCCAGATCTCAGAGGGAAAGCAACAGAGCGGCCAATGGCTCAGCATAGCAGCCGTCC
  AGAGCTTCTAGAAGCCGAGCCTGGTGGCCTCCTCACCAGCCGAGGTTTCATCCGTCTCCCGATGTTGGGC
  TACATCTACCGTGTGTCCAGTGTAAGCAGTGATGAGATTTGGCTCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_027865
• Insert Size: 1098 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_027865.2, NP_082141.1
• RefSeq Size: 2354 bp
• RefSeq ORF: 1098 bp
• Locus ID: 71687
• UniProt ID: Q9DCF1
• Cytogenetics: 9 A5.2
• Gene Summary: In neurons, modulates the degradation of NMDA receptor GRIN2B subunit. Plays a role in the regulation of neuronal excitability.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (b) is shorter at the N-terminus compared to isoform a. Variants 2, 3, and 4 all encode the same isoform (b).