Kazn (NM_001109684) Mouse Untagged Clone

CAT#: MC211194

Kazn (untagged) - Mouse RIKEN cDNA 9030409G11 gene (9030409G11Rik), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Kazn
• Synonyms: 2310007B04Rik; 9030409G11Rik; AI256607; AI666764; AW061099; Kaz; Kazrin
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC211194 representing NM_001109684
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGGAAGACAATAAGCAGCTCGCGCTCCGCATCGATGGGGCGGTCCAGTCGGCCAGCCAGGAGGTGA
  CCAACCTGCGAGCCGAACTCACGGCCACCAACCGGAGACTGGCGGAATTGAGCGGCGGAGGCGGCGGCCC
  CGGCTCGGGCCCGGGAGCTGCGACCAGCGCCTCGGCAGCAGCGGTGACGGTGGCTGACTCGGCAGTGGCG
  ACCATGGAGAACCATCAGCACGGAGCTCAAGTGCTCCTGCGGGAGGAAGTGGTCCAGCTTCAAGAGGAAG
  TGCACCTTCTCCGGCAGATGAAGGAAATGCTGGCGAAGGACCTGGAGGAGTCACAGGGCGGCAAGTGCTC
  TGAGGTCCTCTCAGCCACTGAGCTCCGGGTGCAGCTGGTACAGAAGGAGCAGGAACTGGCCAGAGCCAAA
  GAAGCCTTGCAAGCCATGAAAGCTGACCGGAAGCGCTTAAAGGGCGAGAAGACTGACCTGGTGAGCCAGA
  TGCAGCAGCTGTACGCCACGCTGGAGAGCCGCGAGGAGCAGTTGCGAGACTTCATCCGCAACTATGAGCA
  GCACCGCAAGGAGAGTGAAGACGCAGTCAAAGCACTGGCCAAGGAGAAGGACCTGCTGGAGCGAGAGAAG
  TGGGAGCTTCGACGGCAGGCCAAGGAAGCCACAGACCATGCTGCGGCCCTGCGCTCCCAGCTGGACCTCA
  AGGACAACCGCATGAAGGAGCTGGAGGCGGAGCTCGCCATGGCCAAGCAGTCCTTAGCCACTCTGACCAA
  GGATGTCCCCAAGAGGCATTCCCTCGCCATGCCCGGTGAGACGGTGCTCAATGGCAACCAGGAATGGGTG
  GTGCAGGCTGACCTCCCACTGACTGCTGCCATCCGGCAAAGCCAACAGACTCTCTACCACTCACACCCTC
  CCCACCCTGCAGACCGGCAAGTCAGGGTGAGCCCCTGCCACTCAAGACAGCCCTCCGTCATCTCTGATGC
  TTCTGCTGCTGAAGGTGACCGGTCATCTACACCAAGCGACATCAACTCCCCAAGACACCGGACACACTCC
  CTCTGCAACGGCGACAGTCCCGGCCCAGTTCAGAAGAGCCTACACAACCCTATTGTACAGTCACTAGAGG
  ATCTTGAAGACCAAAAACGGAAAAAGAAGAAGGAGAAGATGGGATTCGGCTCCATCTCTCGAGTCTTCGC
  CAGAGGGAAGCAACGGAAGTCCCTCGACCCGGGCCTCTTTGATGGTACCGCCCCTGATTATTACATAGAG
  GAAGACGCGGACTGGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001109684
• Insert Size: 1278 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001109684.1, NP_001103154.1
• RefSeq Size: 3841 bp
• RefSeq ORF: 1278 bp
• Locus ID: 71529
• UniProt ID: Q69ZS8
• Cytogenetics: 4 E1
• Gene Summary: Component of the cornified envelope of keratinocytes. May be involved in the interplay between adherens junctions and desmosomes. The function in the nucleus is not known.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2, also known as A) lacks several 3' coding exons and its transcription extends past a splice site that is used in variant 1. These changes result in a novel 3' coding region and 3' UTR, compared to variant 1. The encoded isoform (2) has a shorter and distinct C-terminus, compared to isoform 1.