Tssk4 (NM_027673) Mouse Untagged Clone

CAT#: MC211140

Tssk4 (untagged) - Mouse testis-specific serine kinase 4 (Tssk4), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tssk4
• Synonyms: 1700020B19Rik; 4933424F08Rik; TSK-4; TSSK-4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC211140 representing NM_027673
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGAAGGGAGACACCTCGGAAACAGCATCAGCCACCCCAGCCTATCGCTCTGTCATGGAGGAGTATG
  GTTATGAGGTGGGCAAGATCATTGGCCATGGCTCCTATGGAACTGTCTATGAGGCATACTACACAAAGCA
  GAAGGTCATGGTGGCTGTCAAGATCATCTCGAAGAAGAAGGCCTCTGAAGACTATCTCAACAAGTTCCTA
  CCACGTGAGATACAGGTAATGAAAGTCCTACGGCACAAGTACCTCATCAACTTCTATCAGGCCATTGAGA
  CCACATCCCGAGTATACATCATTTTGGAGCTGGCTCAGGGCGGTGATGTCCTCGAATGGATCCAACGATA
  TGGGGCCTGTGCTGAGACCCTTGCTGGCAAGTGGTTCTCCCAGATGGCTTTGGGCATCGCCTACCTGCAC
  AGCAAGGGCATCGTGCACCGGGATTTAAAGTTGGAGAACCTGTTGCTGGACAAGCGGGAGAATGTGAAGA
  TATCGGACTTTGGCTTCGCCAAGATGGTGCCTTCTAGCCAGCCTGTGCATAGTAGCCCTTCTTACCGCCA
  AATGAACAGCCTTTCCCACCTCAGCCAGACCTACTGTGGCAGCTTTGCTTACGCCTGCCCGGAGATCTTG
  CTAGGCTTGCCCTACAACCCTTTCCTGTCTGACACCTGGAGCATGGGCGTCATCCTCTACACTCTAGTGG
  TTGCACGGCTGCCCTTTGATGACACCAATCTCAAGAAGCTGCTGAGAGAAACCCAGAAGGAGGTCACTTT
  CCCAGCTAACTTGACCATCTCCCAGGAGTGCAAGAACCTGATCCTCCAGCTGCTACGCCAATCTACCAAG
  CGTGCCACCATCCTAGATGTCCTCAGGGACCCCTGGATGCTCAAGTTCCAGCCTGAGCAACCTTCGAATG
  AAATCAGGCTGCTCGAGGCCATGTACCAACCCACCAGCTCTGCTAAACGGCACCAGTCCTTGGAAATCAC
  AACCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_027673
• Insert Size: 987 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_027673.3, NP_081949.1
• RefSeq Size: 1243 bp
• RefSeq ORF: 987 bp
• Locus ID: 71099
• UniProt ID: Q9D411
• Cytogenetics: 14 C3
• Gene Summary: Isoform 1: Serine/threonine kinase which is involved in male germ cell development and in mature sperm function (PubMed:17927909, PubMed:23599433, PubMed:23054012, PubMed:25361759, PubMed:26940607). May be involved in the Cre/Creb signaling pathway (PubMed:26940607). Phosphorylates CREB1 on 'Ser-133' in vitro and can stimulate Cre/Creb pathway in cells (By similarity). Phosphorylates CREM on 'Ser-116' in vitro (PubMed:26940607). Phosphorylates ODF2 on 'Ser-95' (PubMed:26961893).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.