Dcp2 (NM_027490) Mouse Untagged Clone

CAT#: MC211089

Dcp2 (untagged) - Mouse DCP2 decapping enzyme homolog (S. cerevisiae) (Dcp2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Dcp2
• Synonyms: 2410015D23Rik; 5730537H01Rik; AL118268
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC211089 representing NM_027490
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCCCAAACGCCTGGAGATTCCCGGCAGCGTCCTGGATGATCTCTGCAGCCGATTTATTTTGCATA
  TTCCCAGTGAAGAAAGAGACAATGCGATCCGAGTGTGCTTCCAGATTGAACTTGCCCATTGGTTTTACTT
  GGATTTCTACATGCAGAACACACCAGGATTACCTCAGTGCGGGATAAGAGACTTTGCCAAAGCTGTTTTC
  AGTCACTGTCCATTTCTGCTGCCACAAGGTGAAGATGTGGAAAAGATTTTGGATGAATGGAAGGAATATA
  AAATGGGAGTCCCGACTTACGGTGCAATTATTCTCGATGAGACACTGGAGAATGTACTGCTGGTTCAGGG
  GTACCTGGCAAAGTCAGGCTGGGGATTCCCAAAAGGAAAAGTAAATAAAGAAGAGGCGCCTCATGACTGT
  GCGGCGAGAGAGGTCTTCGAGGAAACTGGTTTTGATATAAAAGACTATATTTGTAAGGATGATTACATTG
  AACTGCGAATCAATGACCAGCTTGCTCGCTTGTACATCATTCCAGGAGTCCCAAAAGACACAAAATTTAA
  CCCCAAAACCAGAAGAGAAATTAGGAATATTGAGTGGTTCTCCATTGAGAAATTGCCCTGTCATAGAAAT
  GACATGACTCCCAAGTCCAAGCTTGGCTTGGCACCTAATAAGTTTTTTATGGCCATTCCCTTTATCAGAC
  CACTAAGGGACTGGCTGTCTCGAAGATTTGGAGATTCCTCGGACAGTGACAATGGGTTTTCCTCAGCTGG
  TAGCACACCAGCTAGACCCACCGTGGAGAAATTGAGTCGAACAAAATTCCGCCACAGCCAGCAGCTGTTT
  CCTGAAGGTTCCCCGAGTGACCAGTGGGTTAAGCACCGGCAGCCACTGCAGCAGAAGTCACACAGTAACC
  ACGGGGAGGTGTCGGACCTTCTCAAAGCAAAGAATCAAAATATGAGAGGGAATGGCAGAAAACAGTATCA
  AGACTCACCTAATCAAAAGAAGAGAGCGAACGGAGTCCATGGTCAGCCGGCAAAGCAGCAGAATCCCTTG
  GTGAAATGTGAAAAGAAGCTGCATCCACGGAAACTTCAGGACAACTTCGAGACAGATGCCACATGTGACC
  TGCCTTGTTCTGGTGAAGAGCCGTCGGTAGAACATGCTGAGGGACATTCTGTGGCATGCAACGGACATTG
  CAAGTTCCCATTTTCATCCAGAGCCTTTCTGAGTTTCAAGTTTGACCAAAATGCTATAATGAAAATCTTG
  GACCTTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_027490
• Insert Size: 1269 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_027490.1, NP_081766.1
• RefSeq Size: 8643 bp
• RefSeq ORF: 1269 bp
• Locus ID: 70640
• UniProt ID: Q9CYC6
• Cytogenetics: 18 B3
• Gene Summary: Decapping metalloenzyme that catalyzes the cleavage of the cap structure on mRNAs (PubMed:21070968). Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP (PubMed:21070968). Necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay (By similarity). Plays a role in replication-dependent histone mRNA degradation (By similarity). Has higher activity towards mRNAs that lack a poly(A) tail (PubMed:21070968). Has no activity towards a cap structure lacking an RNA moiety (PubMed:21070968).[UniProtKB/Swiss-Prot Function]