Draxin (NM_027426) Mouse Untagged Clone

CAT#: MC211068

Draxin (untagged) - Mouse RIKEN cDNA 2610109H07 gene (2610109H07Rik), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Draxin
• Synonyms: 2610109H07Rik; neucrin
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC211068 representing NM_027426
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGGGTGCCCCGTCCTCAGGGTCCCCACGCTGTTCCTGATCCTCCTGCTGTTTCCAGAGCTCCACA
  CGGCAGGCACCCTTGCATCTGGATCCTCTGCCCGGAACCTGCCGGAGACCCACTCCCACCTCCCCAGCTC
  TGCACTGTGGGTGTCCCAGGCAAGCCATCATGGCCGTCGGGGCCTGGGGAAGAAAGACAGGGGCCCAGGA
  AGGCCTAGCCGGGCCCAGGAGGGGGCTGTGGTCACTGCTACCAAGCAGGCTTCCCAGATGACACTCGGAC
  AGCCCCCTGCTGGCCTTCTGCAGAATAAGGAGCTGCTTCTGGGGCTGACTTTGCCCTACCCCGAGAAGGA
  GGCCCGGTCTCCCGCTTGGGAGAGGGTGAAGAAACGTGGCAGAGAACACAAGAGACGCAGGGACCGTCTG
  CGACTGCACCGAGGCCGAGCTGCCATCCGTGGCCCCAGCTCCCTCATGAAGAAGGTGGAACCCTCTGAAG
  ACCGGATGCTGGAGGGTACCATGGAGGAGTCTTCCACTAGCCTGGCCCCCACCATGTTCTTCCTGACCAT
  GACAGACGGTGCCACGCCTACTACAGAAGAGTCCCGGATCCTGCCTGTCACGTCCTTGCGGCCCCAGACA
  CAGCCCAGGTCTGACGGGGAGGTGATGCCCACACTGGACATGGCCTTATTTGACTGGACGGATTATGAAG
  ACTTAAAGCCAGAGGTCTGGCCTTCTGCAAAGAAGAAAGAGAAACACTGGAGTCATTTTACCAGTGATGG
  TAACGAGACCTCGCCAGCTGAGGGGGATCCGTGTGACCATCACCAGGATTGCTTGCCAGGAACTTGCTGT
  GACCTCCGGGAACATCTCTGCACACCCCACAACCGCGGCCTCAACAACAAATGTTTCGACGACTGCATGT
  GCATGGAAGGGCTGCGTTGCTATGCCAAATTCCACCGGAACCGCAGGGTCACTCGGAGGAAGGGGCGCTG
  CGTGGAACCGGAGACAGCCAACGGGGACCAGGGATCTTTCATCAACATCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_027426
• Insert Size: 1032 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_027426.3, NP_081702.2
• RefSeq Size: 5217 bp
• RefSeq ORF: 1032 bp
• Locus ID: 70433
• UniProt ID: Q6PAL1
• Cytogenetics: 4 E1- E2
• Gene Summary: Chemorepulsive axon guidance protein required for the development of spinal cord and forebrain commissures. Acts as a chemorepulsive guidance protein for commissural axons during development. Able to inhibit or repel neurite outgrowth from dorsal spinal cord. Inhibits the stabilization of cytosolic beta-catenin (CTNNB1) via its interaction with LRP6, thereby acting as an antagonist of Wnt signaling pathway.[UniProtKB/Swiss-Prot Function]