Aunip (NM_001081099) Mouse Untagged Clone

CAT#: MC211019

Clhc1 (untagged) - Mouse RIKEN cDNA 2610002D18 gene (2610002D18Rik), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Aunip
• Synonyms: 2610002D18Rik; Clhc1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC211019 representing NM_001081099
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGCATAGAGGGCCCGAGGAGGAGGCGTGTGGTGTGTGGTTGGACGCGGCGGCGCTGAAGAGGCGGA
  AGATGCAGACACATTTAATCAAACTAGGCACCAAAATGCTGCCATTGCTTCCTGGAGAGAGGAAACCTAA
  TGATCCTTTTACCCAAAGAAGAGGCACTAGACAGACCAGCATTGCATCTTTTGTCACGCTACAATCAGGA
  ATGGCAAGTGGTGGTAACCAGAAGAACATTTTCTCTCTCAAAGAAAATCAGACCAACAAAGAATGCAAGA
  GAACCCAGCTAGACTGTTTGGATGATGGCTTGCTATCACCTTTGGTCACTTCAGCTCCTGCAGACATCCA
  GGAAGCTGGGCATCCTCGGTCTCCCCAGATTTCAGGCTGCCAGGGTTTGGAAATGTCATCTTTGACTATG
  ATGTCTTTGACCCAGCCTGATGTCCTGATGGGCACTGGAGAGAGTAAAGGCCCCCTGGATTCTTCCTTTT
  CCCAATACTTGGAACGTTCTTGTTTGCTGGACCAAAGAGAGGCCAAGAGGAAAGGGGAAGGGCTTCGTGA
  ATCTAAGACAGACTGTCCAGGGATGGGAAGCCACATCAGACCACCGGGGAGTAAATGCCATCAGCCCTTG
  GACAAAGCTGAAATGGGCAAGAGGGGGCCTACCAAGGAAAACAGGCAGGCCCCTGTGCATCTTCAGACCT
  ATAGATCTGGATCCTGCAGTAGGAAAAAAACACTATTGGTGACAGAAAGCCCTTGTCCTCTTTCCTTATT
  TCCCTGGGACAGTGAAAGGAGTGACAGGGACTCCTGGAGTCAGCTTTTCACTGAGGATTCACAAGGCCAG
  CAAGTCATTGCCCACAACACTAAAATGCCTTTCCGAGATGTGACCAATGCTAGGAATCAAGGCTCAGGGC
  AGTTTCCTGACAGCCCACAGGCTCAGGGCCAAGACGGGCCTGCTCAGTTACATCTGCAGTCATACCTGCT
  CTTTACCCAGGACTCTGAGGGTAATCGGGTTATCAGGCACTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001081099
• Insert Size: 1023 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001081099.1, NP_001074568.1
• RefSeq Size: 1270 bp
• RefSeq ORF: 1023 bp
• Locus ID: 69885
• UniProt ID: E9Q6Z5
• Cytogenetics: 4 D3
• Gene Summary: DNA-binding protein that accumulates at DNA double-strand breaks (DSBs) following DNA damage and promotes DNA resection and homologous recombination. Serves as a sensor of DNA damage: binds DNA with a strong preference for DNA substrates that mimic structures generated at stalled replication forks, and anchors RBBP8/CtIP to DSB sites to promote DNA end resection and ensuing homologous recombination repair. Inhibits non-homologous end joining (NHEJ). Required for the dynamic movement of AURKA at the centrosomes and spindle apparatus during the cell cycle.[UniProtKB/Swiss-Prot Function]