Ddi2 (NM_001017966) Mouse Untagged Clone

CAT#: MC210796

Ddi2 (untagged) - Mouse DNA-damage inducible protein 2 (Ddi2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ddi2
• Synonyms: 1110056G13Rik; 1700027M01Rik; 9130022E05Rik; AI604911; AU040698
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC210796 representing NM_001017966
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCTCACCGTGTACTGTGTGCGGAGGGACCTCTCCGAGGTGACCTTTTCCCTCCAGGTCGACGCCG
  ACTTCGAGCTGCACAACTTCCGCGCGCTGTGCGAGCTCGAGTCCGGCATCCCCGCCGCCGAGAGCCAGAT
  TGTCTATGCAGAAAGGCCTCTCACCGACAACCACAGGTCACTGGCTTCTTACGGTTTAAAAGATGGTGAT
  GTTGTGATTCTACGACAGAAGGAGAATGCAGACCCTCGCCCTGCAGTGCAGTTCTCAAACTTACCCCGGA
  TTGACTTCAGTAGCATAGCTGTGCCTGGCACGTCAAACCCCCAACAGCGCCAGCTGCCCAGAACCCAGGC
  TCAGCACTCATCTCCTGGGGAAATGGCATCTTCTCCTCAAGGCCTAGACAACCCAGCCCTGCTCCGAGAC
  ATGCTGCTGGCCAACCCGCATGAGTTGTCCTTGCTGAAGGAGCGCAACCCACCCTTGGCGGAAGCTCTGC
  TCAGTGGAGATCTTGAGAAATTCTCTAGGGTCTTGGTGGAGCAGCAGCAGGACCGAGCCCGGAGAGAACA
  AGAACGGATTCGTCTGTTTTCTGCTGACCCCTTTGATCTCGAAGCTCAGGCAAAGATAGAAGAAGATATA
  AGGCAGCAGAACATCGAGGAGAACATGACGATAGCTATGGAGGAGGCCCCGGAGAGCTTCGGCCAGGTCG
  CCATGCTGTACATCAACTGCAGAGTGAACGGGCATCCTGTGAAAGCCTTTGTCGACTCAGGAGCCCAGAT
  GACTATTATGAGCCAGGCCTGTGCAGAAAGGTGTAACATAATGAGACTGGTAGATCGTCGGTGGGCTGGC
  ATTGCCAAAGGAGTAGGCACACAGAAGATTATTGGAAGGGTGCATCTAGCTCAGGTTCAGATTGAAGGCG
  ATTTTCTGGCGTGCTCCTTCTCAATTCTGGAAGAACAGCCTATGGACATGCTTCTGGGTCTGGACATGCT
  GAAACGGCACCAGTGTTCTATTGACTTGAAGAAAAATGTTCTGGTGATTGGCACCACGGGCTCACAGACC
  ACCTTCCTTCCTGAGGGGGAGTTACCAGAGTGTGCTCGTTTGGCATATGGAACCGGGCGAGAGGACATAC
  GGCCAGAGGAAATTGCAGACCAAGAATTAGCAGAAGCCATTCAAAAATCAGCCGAGGATGCAGAGCGTCA
  GAAGCCATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001017966
• Insert Size: 1200 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001017966.2, NP_001017966.1
• RefSeq Size: 3539 bp
• RefSeq ORF: 1200 bp
• Locus ID: 68817
• UniProt ID: A2ADY9
• Cytogenetics: 4 E1
• Gene Summary: Aspartic protease that mediates the cleavage of NFE2L1/NRF1 at 'Leu-104', thereby promoting release of NFE2L1/NRF1 from the endoplasmic reticulum membrane. Ubiquitination of NFE2L1/NRF1 is a prerequisite for cleavage, suggesting that DDI2 specifically recognizes and binds ubiquitinated NFE2L1/NRF1. Seems to act as a proteasomal shuttle which links the proteasome and replication fork proteins like RTF2. Required, with DDI1, for cellular survival following replication stress. Together or redudantly with DDI1, removes RTF2 from stalled forks to allow cell cycle progression after replication stress and maintains genome integrity.[UniProtKB/Swiss-Prot Function]