Spindoc (NM_001167831) Mouse Untagged Clone

CAT#: MC210710

AI846148 (untagged) - Mouse expressed sequence AI846148 (AI846148), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Spindoc
• Synonyms: 1700105P06Rik; AI846148; SPIN-DOC
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC210710 representing NM_001167831
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCTGAAAGCCAAGGGCGCCGCGGCTCTGGATTGCTTCGAGGTGACGCTCAAGTGTGAGGAAGGGG
  ATGATGACGACGAGGCCGTGGTGGTGGCCGTGATCCCGCGGCCCGAGCCGATGCTCCGAGTAACCCAACA
  GGAAAAGACGCCGCCACCTCGGCCTAACCTGCTGGAAGCAGGTGTTGAAGGCTGTGAGGAGCTCAAGCAG
  CAAGTGTCATGGGAGCAGGAGTTCCTGGTGGGGAACAGCCCAGGAGGCAGTGGACGGGCGCTGTGCATGG
  TGTGTGGGGCTGAGATCCGGTCCCCATCAGCAGACACTGCTCGCGCACACATCCTGGAGCAGCATCCTCA
  CACCCTGGACCTGAGCCCCTCTGAGAAGAGCAACATCTTAGAAGCCTGGAGTGAAGGGGTTGCCCTTCTG
  CAAGACATCCAAGCTGACCAGCCATCCCTGCCCAGCCTAGAGTCAGGCCAGGATGGCCAGCCAGACCCCA
  TCTCCAACCCGGATCCTGTCAGGATGCCAGCAGAGATAGTGGTCCTCTTGGATTCCGAGGACAACCCATC
  CCTTCCTAAGAGGCTCCGGCCCAGAGGACTCCGCCCCCTTGAGCTGCCCGTTACCCCTGTCATAGAGCAA
  GGGAATAAAAAGCCCCGTGGTCAGAGATGGAAAGAGTCCCCTGAGAATGAACCTGCTAGAAAGAAGAGAA
  GCAGGCATATGACCAAAATCTTGGACCCTGACCCAGACCCCCCGTCTCCTGAGTCACCCACAGAGACATT
  CGCAGCACCAGCTGAAGTCCGACACTTCACTGATGGCAGCTTCCCTCCTGGCTTCGTCCTCCAGCTCTTC
  TCCCACACTCAGCTCAGGACCACTGACTGTAAGGACTCTTCTAAGGACAGCAGAGCAGCAGAGGGCCTGC
  CCCAGCCACAAAACCCCTCTTCAGGTGAGCCCTGCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001167831
• Insert Size: 948 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001167831.1, NP_001161303.1
• RefSeq Size: 1594 bp
• RefSeq ORF: 948 bp
• Locus ID: 68229
• UniProt ID: Q05AH6
• Cytogenetics: 19 A
• Gene Summary: Negatively regulates the transcriptional activator activity of SPIN1 via inhibition of its histone methyl-binding ability. Represses the expression of a number of SPIN1-regulated genes and the SPIN1-mediated activation of the Wnt signaling pathway (PubMed:29061846). Can also inhibit the histone methyl-binding abilities of SPIN2A, SPIN2B, SPIN3 and SPIN4 (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 3' UTR and coding sequence compared to variant 1. The resulting isoform (2) has a shorter and distinct C-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.