Kmt5a (NM_030241) Mouse Untagged Clone

CAT#: MC210666

Setd8 (untagged) - Mouse SET domain containing (lysine methyltransferase) 8 (Setd8), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Kmt5a
• Synonyms: 2410195B05Rik; AA617402; AW536475; PR-SET7; PR/SET07; Setd8
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC210666 representing NM_030241
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTAGAGGCAGGAAGATGTGCAAGCCCCGCGCGGTGGAGGCGGCGGCGGCGGCGGTGGCGGCGACGG
  CCCCGGGCCCGGAGATGGTGGAGCAGAGGGGCCCGGGGAGGCCCCGCAGCGACGGGGAGAACGTGTTTGC
  TGGGCAGTCAAAGATCTATGCCTACATGAGTCCGAACAAGTGCTCTGCAATGCGCTCTCCACTTCAGGAA
  GAGAACTCGGTTGCCCATCATGAGGTCAAATGCCCGGGGAAACCATTAGCTGGAATCTACAGGAAGCGAG
  AAGAGAAAAGGAACACCGGGAACGTTATACGAAGCGCTGTGAAGTCAGATGAACAGAAGAGCAAAGACAC
  CAGGAGAGGTCCCCTGGCGCCTTTTCCAAACCAAAAATCCGAAGCAGCAGAACCTCCAAAAACTCCACCC
  CCATCATGTGATTCTACCAATGTAGCAGTCGCTAAGCAAGCCCTGAAAAAGTCCCTCAAGGGCAAACAGG
  CCCCTCGGAAAAAGTCTCAAGGGAAAACCCAGCAGAATAGAAAACTCACAGATTTCTACCCTGTGCGGAG
  GAGTTCCCGGAAGAGCAAAGCTGAGCTGCAGTCTGAAGAAAGGAAGAAAATAGATGAGCTGATTGAGAGC
  GGGAAGGAAGAAGGCATGAAGATTGATCTAATTGATGGCAAAGGCAGGGGTGTGATCGCTACCAAGCAGT
  TCTCCAGGGGAGACTTTGTGGTAGAATACCATGGGGACCTCATTGAAATCACTGATGCCAAGAAGCGGGA
  GGCTCTGTACGCACAGGACCCCTCCACTGGCTGCTACATGTACTATTTTCAGTATCTGAGCAAAACCTAC
  TGCGTGGATGCCACTCAAGAAACGAACCGCCTAGGGAGACTCATCAATCACAGTAAGTGTGGGAACTGCC
  AGACCAAACTGCACGACATCGACGGCGTGCCTCACCTCATCCTCATCGCCTCCCGAGACATCGCAGCTGG
  GGAGGAGCTCCTGTACGACTATGGGGACCGAAGCAAGGCCTCCATCGAAGCCTACCCCTGGCTGAAGCAC
  TAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_030241
• Insert Size: 1053 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_030241.3, NP_084517.2
• RefSeq Size: 2738 bp
• RefSeq ORF: 1053 bp
• Locus ID: 67956
• UniProt ID: Q2YDW7
• Cytogenetics: 5 F
• Gene Summary: Protein-lysine N-methyltransferase that monomethylates both histones and non-histone proteins. Specifically monomethylates 'Lys-20' of histone H4 (H4K20me1). H4K20me1 is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. Required for cell proliferation, probably by contributing to the maintenance of proper higher-order structure of DNA during mitosis. Involved in chromosome condensation and proper cytokinesis. Nucleosomes are preferred as substrate compared to free histones. Mediates monomethylation of p53/TP53 at 'Lys-382', leading to repress p53/TP53-target genes. Plays a negative role in TGF-beta response regulation and a positive role in cell migration (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes isoform 1.