Naa20 (NM_026425) Mouse Untagged Clone

CAT#: MC210645

Naa20 (untagged) - Mouse N(alpha)-acetyltransferase 20, NatB catalytic subunit (Naa20), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Naa20
• Synonyms: 1500004D14Rik; 2900026I01Rik; AU041458; D2Ertd186e; Nat5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC210645 representing NM_026425
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCACGCTCCGGGCCTTCACCTGCGACGACCTGTTCCGCTTCAACAACATTAACTTGGATCCACTTA
  CAGAAACTTATGGGATTCCTTTTTACTTACAGTACCTTGCGCACTGGCCAGAGTATTTCATCGTCGCTGA
  AGCACCTGGCGGAGAACTAATGGGTTATAGTAAGTACAGTACCACTAGTACTTTTTTGGTTATGGGCAAA
  GCAGAAGGCTCGGTAGCTAGGGAAGAATGGCATGGACATGTCACAGCTCTCTCTGTCGCCCCTGAATTCC
  GCCGTCTTGGCTTGGCTGCTAAACTTATGGAGTTACTAGAAGAAATTTCAGAAAGAAAAGGTGGATTTTT
  TGTTGATCTCTTCGTAAGAGTATCCAATCAAGTTGCCGTCAACATGTACAAGCAGTTGGGTTACAGTGTC
  TACAGGACAGTCATAGAGTACTACTCAGCCAGCAATGGGGAGCCTGATGAGGATGCCTATGATATGAGGA
  AAGCACTTTCCAGGGACACTGAGAAGAAATCCATCATCCCATTACCTCATCCCGTGAGGCCTGAGGACAT
  TGAATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_026425
• Insert Size: 567 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_026425.3, NP_080701.1
• RefSeq Size: 1197 bp
• RefSeq ORF: 567 bp
• Locus ID: 67877
• UniProt ID: P61600
• Cytogenetics: 2 71.28 cM
• Gene Summary: Catalytic subunit of the NatB complex which catalyzes acetylation of the N-terminal methionine residues of peptides beginning with Met-Asp, Met-Glu, Met-Asn and Met-Gln. Proteins with cell cycle functions are overrepresented in the pool of NatB substrates. Required for maintaining the structure and function of actomyosin fibers and for proper cellular migration.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) uses an in-frame alternate splice site in the coding region, compared to variant 1, and encodes a longer protein (isoform 2), compared to isoform 1.