Brf2 (NM_025686) Mouse Untagged Clone

CAT#: MC210411

Brf2 (untagged) - Mouse BRF2, subunit of RNA polymerase III transcription initiation factor, BRF1-like (Brf2), (10ug)


  "NM_025686" in other vectors (4)

Reconstitution Protocol

USD 503.00

4 Weeks*

Size
    • 10 ug

Product Images

Frequently bought together (3)
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Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
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Other products for "Brf2"

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Symbol Brf2
Synonyms 2700059M06Rik; 5730512K07Rik; AU018504; AU022602; BRFU; TFIIIB50
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC210411 representing NM_025686
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCGAACGGAAGCCGCTGCCCGGATTGCGGCTCCTCCGAGCTCGTGGAAGATTCGCATTATTCTCAGA
GCCAGCTGGTGTGCTCCGACTGCGGCTGCGTGGTCACCGAAGGGGTCCTAACTACCACCTTTAGCGACGA
GGGCAACTTCAGGGAAGTGACATATTCTCGAAGCACTGGGGAAAACGAACAAGTTAGTCGCTGCCAGCAA
CGAGATCTCCGTCGAGTAAGAGACCTGTGTCGGATTCTGAAGCTGCCGCTAACGTTTGAGGATACAGCAA
TCTCCTACTACCAGAAGGCATATCAGCTGTCTGGGATCCGAGCTGCCAGGCTGCAGAAGAAGGAAGTGTT
AGTCGGATGCTGTGTTTTAATCACCTGTCGGCAACATAACTGGCCCCTCACCATGGGAACCATCTGCACA
CTGCTCTATGCCGATCTGGACTTGTTTTCTGGCACCTACATGCAGATGGTAAAGCTCCTGGGGCTGGATG
TGCCCTCCCTGTGCCTGGCAGACTTGGTGAAGTCTTACTGCAGCAGCTTCAAACTGTTCCAAGCGTCCCC
GTCCGTGCCAGCCAAATACGTGGAAGACAAAGACAAGATGCTGTCTCGAACCTTGCTGTTGGTGGAGCTA
GCAGATGAGACGTGGCTAGTGACTGGACGGCATCCCTTGCCCATCATCACTGCAGCCACCTTCCTGGCAT
GGCAGTCTCTGCGGCCTTCAGACCGGCTGACCTGCTCTCTTGCCCAGTTTTGTAAATTGGCGAATGTGGA
CCTGCCCTACCCAGCTGCCTCTCGCCTGCAGGAGCTCCTGGCCGTGCTCCTTCAGATGGCCGGCCAGCTG
GCCTGGCTACAGGTTCTAAAACTTAACAAGCGGTCGGTGGTGAAACACATTGGTGACCTTCTCCAGCACC
GCCACATGCTGGTCCGAACGGCTTTTCGAGATGGAACAGCAGAAGTGGAGACCCAGCAGCAGCAGCAGCA
GCAGCAGGGACAAGGACAAGGACAGCAGGACGAGGTGGGGGATGGTCCCTTCGATTTGCCCAAGAGGAAA
CGTCCCGCCAGTCCCACTCCCCTCCTGCCCCCCTGCATGTTGAAGCCCCCCAAGCGGACCCACACCTTGC
CCCCCGAATCTGCAGTGACTGGCGATGAGGATATTTCAGATAGTGAAATAGAGCAGTATTTGCGCACCCC
TCAGGAAGTTAGAGACTTTGAGAGAGCCCAAGCTGCTAGCCAGGCAGCCATGCGTGTCCCTAACCCTCCC
TGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_025686
Insert Size 1263 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_025686.2, NP_079962.1
RefSeq Size 1383 bp
RefSeq ORF 1263 bp
Locus ID 66653
UniProt ID Q3UAW9
Cytogenetics 8 A2
Gene Summary General activator of RNA polymerase III transcription. Factor exclusively required for RNA polymerase III transcription of genes with promoter elements upstream of the initiation sites. Contributes to the regulation of gene expression; functions as activator in the absence of oxidative stress. Down-regulates expression of target genes in response to oxidative stress. Overexpression protects cells against apoptosis in response to oxidative stress.[UniProtKB/Swiss-Prot Function]

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.