Taf8 (NM_022015) Mouse Untagged Clone

CAT#: MC210215

Taf8 (untagged) - Mouse TAF8 RNA polymerase II, TATA box binding protein (TBP)-associated factorq (Taf8), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Taf8
• Synonyms: AW260255; Tbn
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC210215 representing NM_022015
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGACACGGCGGCCGGGCCTGGTGGCTCCGGAACGAGACCGGGAAGTAAGCAATCCACTAACCCTG
  CTGATAACTACCATCTGGCCCGGAGGAGGACCCTGCAAGTGGTTGTGAGCTCCTTGCTGACAGAGGCGGG
  ATTTGAGAGTGCCGAGAAAGCGTCTGTGGAGACACTGACAGAGATGCTGCAGAGCTACATCTCAGAAATC
  GGGAGAAGTGCTAAGTCATACTGTGAGCACACAGCCAGGACCCAGCCCACACTGTCGGACATCGTGGTCA
  CATTGGTTGAAATGGGCTTCAATGTTGACACTCTACCTGCCTATGCAAAACGGTCTCAGAGGATGGTCAT
  CACTGCACCTCCAGTGACCAATCAGCCAGTGACCCCCAAGGCCCTCACTGCAGGGCAGAACCGGCCCCAC
  CCGCCACACATCCCCAGCCATTTTCCTGAGTTTCCGGATCCCCACACCTACATCAAAACTCCGACATACC
  GTGAGCCAGTGTCTGACTACCAGATCCTTCGGGAGAAGGCCGCATCTCAGAGACGAGACGTGGAGCGAGC
  ACTCACCCGCTTCATGGCCAAGACAGGCGAGACCCAGAGTCTTTTCAAGGATGATGTCAGCACTTTTCCT
  TTGATCGCTGCCAGACCGTTCACCATCCCCTACCTCACGGCCTTGCTTCCTTCTGAGCTGGAGATACAGC
  AGATGGAAGAGACCGACTCCTCGGAGCAGGAGGAGCAAACAGATACAGAGAACAACGCTCTTCATATCAG
  CACGGACGATTCCGGAGCTGAGAAGGAGAGCGCTTCTGTTCTGCAGCAGAGCTCCTCCTTGTCCGGCAGC
  CGGAATGGCGAGGAGAGCGTCATCGATAACCCCTATCTGCGGCCCGTGAAGAAGCCCAAGATCCGTAGGA
  AGAAGTCCCTCTCGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_022015
• Insert Size: 927 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022015.3, NP_071298.1
• RefSeq Size: 2779 bp
• RefSeq ORF: 927 bp
• Locus ID: 63856
• UniProt ID: Q9EQH4
• Cytogenetics: 17 C
• Gene Summary: Transcription factor TFIID is one of the general factors required for accurate and regulated initiation by RNA polymerase II. Mediates both basal and activator-dependent transcription. Plays a role in the differentiation of preadipocyte fibroblasts to adipocytes, however does not seem to play a role in differentiation of myoblasts. Required for the integration of TAF10 in the TAF complex (By similarity). May be important for survival of cells of the inner cell mass which constitute the pluripotent cell population of the early embryo.[UniProtKB/Swiss-Prot Function]