Mocs1 (NM_028464) Mouse Untagged Clone

CAT#: MC210090

Mocs1 (untagged) - Mouse molybdenum cofactor synthesis 1 (Mocs1), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mocs1
• Synonyms: 3110045D15Rik; AI043170; AW536397
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC210090 representing NM_028464
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGCGGCCTGCGTTCGGAATAGTGCGGCAGCTCCTGAGGTCCAATGCTCGGGGTTGCAGCTCGG
  GGGCTCCGGTGACGCAGCCTCGCCCCGGGGAGCCCTCGAGACCTACCAGGGAGGGGCTGTCCCTGAGGCT
  CCAGTTCCTCCAGGAGCATGCGGCTCCCTTCTCTGCCTTCCTCACGGACAGCTTTGGCCGACAACATAGT
  TACCTGCGGATCTCTCTCACAGAGAAGTGCAACCTCCGATGCCAGTATTGCATGCCCGAGGAGGGTGTGC
  CCCTGACCCCCAAAGCTGACCTACTGACCACAGAGGAGATCCTGACCCTTGCCCGCCTCTTTGTGAAGGA
  AGGTGTGGACAAGATCCGTCTCACTGGTGGGGAGCCTCTCATCCGACCGGATGTGGTGGACATCGTGGCC
  AGACTTCATGGGCTGGAAGGGCTGAGAACCATCGGCCTCACCACCAACGGCATCAACCTAGCCCGGCTGC
  TGCCCCGACTTCAGCAAGCAGGGCTCAATGCTGTTAACATCAGCCTGGACACTCTGGTTCCCGCCAAGTT
  TGAGTTCATCGTCCGCAGAAAAGGCTTCCACAAGGTTATGGAGGGTATCCACAAAGCCATCGAGCTGGGT
  TACAAGCCTGTGAAGGTGAACTGTGTGGTGATGCGAGGCCTGAATGAAGACGAGCTGCTGGACTTCGTGG
  CCCTGACAGAGGGCCTTCCCTTGGACGTGCGCTTCATTGAGTACATGCCATTTGACGGCAACAAGTGGAA
  CTTCAAGAAGATGGTTAGCTACAAGGAGATGCTGGACACCATCCGACAGCGGTGGCCAGGGCTGGAGAAG
  CTGCCGGAGGAGGACTCGAGCACTGCCAAGGCCTTTAAGATCCCTGGCTTCCAGGGTCAGATCAGCTTCA
  TCACATCCATGTCAGAGCATTTCTGTGGGACCTGCAACCGGTTGCGCATCACAGCTGATGGGAACCTCAA
  GGTCTGCCTCTTTGGGAACTCTGAGGTGTCACTGCGGGACCACCTGCGGGCGGGGGCCTCTGAGGAGGAG
  CTGCTGAGAATCATTGGGGCCGCAGTGGGTAGGAAGAAGCGGCAGCACGCAGGCATGTTCAATATTGCCC
  AGATGAAGAACCGGCCCATGATCCTCATCGGTGGGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_028464
• Insert Size: 1158 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_028464.1, NP_082740.1
• RefSeq Size: 2655 bp
• RefSeq ORF: 1158 bp
• Locus ID: 56738
• UniProt ID: Q5RKZ7
• Cytogenetics: 17 C
• Gene Summary: Isoform Mocs1a and isoform Mocs1b probably form a complex that catalyzes the conversion of 5'-GTP to cyclic pyranopterin monophosphate (cPMP). Mocs1a catalyzes the cyclization of GTP to (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate and Mocs1b catalyzes the subsequent conversion of (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate to cPMP.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) uses a different splice site in the 3' UTR which results in an early stop codon, compared to variant 1. The resulting protein (isoform 2 which was also called protein A) has a shorter and distinct C-terminus when it is compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.