Hs6st1 (NM_015818) Mouse Untagged Clone

CAT#: MC209866

Hs6st1 (untagged) - Mouse heparan sulfate 6-O-sulfotransferase 1 (Hs6st1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Hs6st1
• Synonyms: 6Ost1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC209866 representing NM_015818
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTTGAGCGCGCCAGCAAGTTCGTGCTGGTGGTGGCGGGCTCGGCGTGCTTCATGCTCATCCTTTACC
  AGTACGCGGGCCCGGGGCTGAGTCTGGGCGCGCCCGGTGGCCGCGTGCCCCCCGACGACCTGGATCTCTT
  CCCCACGCCGGACCCACATTACGAGAAAAAGTACTACTTCCCGGTGCGCGAGCTGGAGCGCTCGCTGCGC
  TTCGACATGAAGGGCGACGACGTGATCGTCTTCCTGCACATCCAGAAGACCGGCGGCACCACCTTCGGCC
  GCCACCTAGTGCAGAACGTGCGCCTCGAGGTGCCCTGCGACTGTCGCCCGGGCCAGAAGAAGTGCACCTG
  CTATCGGCCCAATCGCCGCGAGACCTGGCTCTTCTCTCGCTTCTCCACGGGCTGGAGCTGCGGGCTGCAC
  GCTGACTGGACCGAACTCACCAACTGTGTGCCCGGTGTGCTAGACCGCCGCGACCCAGCAGGTCTGCGTT
  CGCCCAGAAAGTTCTACTACATCACCCTGCTGCGAGACCCCGTATCCCGCTACCTGAGTGAATGGCGACA
  TGTACAGCGTGGGGCCACGTGGAAGACCTCCTTGCACATGTGTGACGGGCGCACACCGACCCCAGAGGAG
  CTGCCGCCCTGCTACGAGGGCACAGACTGGTCGGGCTGCACGTTGCAGGAGTTCATGGATTGCCCCTATA
  ACCTGGCTAACAACCGGCAGGTGCGCATGCTGGCCGACCTCAGCCTGGTGGGCTGCTACAACCTATCTTT
  CATCCCCGAGAGCAAGCGGGCCCAGTTGCTGCTGGAGAGCGCCAAGAAGAACCTGCGAGGCATGGCCTTC
  TTCGGCCTCACTGAGTTCCAGCGCAAGACGCAGTACCTATTTGAGCGGACGTTCAACCTCAAGTTCATCC
  GGCCATTCATGCAATACAACAGCACGCGGGCGGGCGGTGTGGAGGTGGATGAGGACACTATCCGCCACAT
  CGAGGAGCTCAACGACCTGGACATGCAGCTGTATGACTATGCCAAGGACCTCTTTCAGCAGCGTTACCAG
  TACAAGAGACAGCTGGAGCGCAGGGAACAGCGCCTGCGCAATCGCGAAGAGCGCCTCCTGCACCGCTCCA
  AGGAAGCGCTGCCACGGGAGGACCCAGAAGAGCCGGGCCGTGTGCCCACCGAGGACTACATGAGCCATAT
  CATTGAGAAGTGGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_015818
• Insert Size: 1206 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015818.2, NP_056633.2
• RefSeq Size: 3732 bp
• RefSeq ORF: 1236 bp
• Locus ID: 50785
• UniProt ID: Q9QYK5
• Cytogenetics: 1 B
• Gene Summary: 6-O-sulfation enzyme which catalyzes the transfer of sulfate from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to position 6 of the N-sulfoglucosamine residue (GlcNS) of heparan sulfate. Critical for normal neuronal development where it may play a role in neuron branching. May also play a role in limb development. May prefer iduronic acid.[UniProtKB/Swiss-Prot Function]