Rcn2 (NM_011992) Mouse Untagged Clone

CAT#: MC209742

Rcn2 (untagged) - Mouse reticulocalbin 2 (Rcn2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rcn2
• Synonyms: AA408742; Tcbp49
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC209742 representing NM_011992
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGGCTGGGCCCAAGGCCCGCGGCGCTAGGACTGCTGCTGCCGCTGCTGCTGTACGCCGCGGTGGCTG
  GCGCCAGCAAGGCGGAGGAACTGCACTACCCGCAGGGCGAGCACCGGGCGGACTACGACCGCGAAGCGCT
  GCTGGGTGTCCAGGAAGACGTCGATGAGTATGTTAAACTTGGCCACGAAGAGCAGCAAAGACGATTGCAG
  TCGATCATAAAGAAAATTGACTCGGACTCTGATGGCTTTCTTACTGAAAATGAACTCAGTCAGTGGATTC
  AGATGTCTTTTAAGCATTACGCTATGCAAGAAGCCAAGCAGCAGTTTGTGGAGTATGATAAGAACAGCGA
  CGGCGCTGTGACGTGGGATGAGTACAACATCCAGATGTACGACCGGGTGATTGACTTTGATGAGAACACT
  GCTCTGGATGACACAGAAGAGGGGTCGTTCAGGCAGCTTCATCTAAAGGATAAGAAGCGATTTGAAAAAG
  CTAACCAGGATTCAGGTCCTGGTCTGAGTCTTGAAGAGTTCATTGCGTTTGAGCACCCTGAAGAAGTTGA
  CTATATGACGGAGTTCGTCATCCAAGAGGCTTTGGAAGAACATGACAAAAATGGCGATGGGTTTGTTAGT
  TTGGAAGAATTTCTTGGCGATTACAGGCGGGATCCAACTGCAAATGAAGACCCAGAATGGATACTTGTTG
  AAAAGGACAGATTTGTGAATGATTATGACAAAGATAATGATGGCCGGCTTGATCCCCAAGAGCTGTTGTC
  ATGGGTAGTGCCCAATAACCAGGGCATTGCACAAGAGGAAGCTCTTCATCTGATTGATGAGATGGATTTG
  AATAGTGACAAAAAGCTCTCTGAAGAAGAGATTCTGGAAAACCAAGACTTGTTCCTTACCAGCGAAGCCA
  CGGATTATGGCCGACAGCTCCATGACGACTACTTCTATCATGACGAGCTTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_011992
• Insert Size: 963 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011992.2, NP_036122.2
• RefSeq Size: 2042 bp
• RefSeq ORF: 963 bp
• Locus ID: 26611
• UniProt ID: Q8BP92
• Cytogenetics: 9 B
• Gene Summary: Not known. Binds calcium (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). CCDS Note: This CCDS representation uses a downstream start codon that is well-conserved in mammalian organisms. It also contains an upstream in-frame start codon that would result in a 35 aa N-terminal extension. However, this upstream start codon is only present in mouse and rat, and its use would eliminate an N-terminal signal peptide that is only predicted in the shorter N-terminus. N-terminal sequencing of the rat protein in PMID:7722520 indicates signal peptide cleavage. It is likely that the upstream start codon is skipped due to leaky scanning by ribosomes, thereby permitting translation initiation at the currently represented start codon.