Map2k3 (NM_008928) Mouse Untagged Clone

CAT#: MC209717

Map2k3 (untagged) - Mouse mitogen-activated protein kinase kinase 3 (Map2k3), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Map2k3
• Synonyms: AW212142; MEK3; MKK3; mMKK3b; Prkmk3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC209717 representing NM_008928
  Red=Cloning site Blue=ORF
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGTCGCCCGCCGCGAGCCCGCCGGCCAGCTTGCCTCAGACCAAAGGAAAATCCAAAAGGAAAAAGG
  ACTTACGGATATCCTGCGTGTCCAAGCCACCTGTGTCCAACCCCACACCCCCCCGGAACCTGGACTCCCG
  GACCTTCATCACTATCGGAGACAGAAACTTCGAAGTGGAGGCTGATGACTTGGTGACCATCTCAGAGCTG
  GGTCGTGGAGCCTATGGGGTGGTAGAGAAAGTGCGGCATGCTCAGAGTGGTACCATCATGGCTGTCAAGC
  GCATCCGGGCCACAGTGAACACACAGGAGCAGAAACGTCTGCTTATGGACCTAGACATCAACATGCGCAC
  GGTCGACTGCTTCTACACTGTCACCTTCTATGGTGCCCTCTTCAGAGAGGGGGATGTATGGATCTGCATG
  GAGCTCATGGACACTTCCCTGGATAAGTTCTACCGGAAGGTGCTGGAGAAGAACATGAAAATTCCGGAAG
  ACATCCTGGGGGAGATCGCTGTGTCTATCGTGCGGGCCCTGGAGCACCTGCATAGCAAGCTGTCTGTGAT
  CCACAGAGATGTGAAGCCATCCAATGTCCTCATCAACAAGGAAGGGCATGTGAAGATGTGCGACTTTGGC
  ATCAGTGGCTACCTGGTGGACTCTGTGGCAAAGACAATGGATGCTGGCTGCAAGCCTTACATGGCCCCTG
  AGAGGATCAACCCTGAACTGAATCAGAAGGGCTACAATGTCAAGTCTGATGTCTGGAGCCTCGGCATCAC
  CATGATCGAGATGGCCATTCTGCGATTCCCTTATGAGTCTTGGGGCACACCGTTCCAGCAGCTGAAGCAG
  GTGGTGGAGGAGCCATCCCCACAGCTCCCAGCGGACCAGTTCTCCCCTGAGTTTGTGGACTTCACTAGCC
  AGTGCCTAAGGAAGAACCCTGCAGAGCGCATGAGCTACCTGGAGCTGATGGAACACCCATTCTTCACCTT
  GCACAAAACTAAGAAGACAGACATTGCTGCCTTTGTGAAGGAGATCCTGGGAGAGGATTCATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_008928
• Insert Size: 1044 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008928.4, NP_032954.1
• RefSeq Size: 2166 bp
• RefSeq ORF: 1044 bp
• Locus ID: 26397
• UniProt ID: O09110
• Cytogenetics: 11 B2
• Gene Summary: Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38. Part of a signaling cascade that begins with the activation of the adrenergic receptor ADRA1B and leads to the activation of MAPK14.[UniProtKB/Swiss-Prot Function]