Wnt11 (NM_009519) Mouse Untagged Clone

CAT#: MC209648

Wnt11 (untagged) - Mouse wingless-related MMTV integration site 11 (Wnt11), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Wnt11
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC209648 representing NM_009519
  Red=Cloning site Blue=ORF
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGGCGCGGCCGCAGGTCTGCGAGGCTCTGCTCTTTGCCTTGGCGCTCCACACCGGCGTGTGCTATG
  GCATCAAGTGGCTGGCACTGTCCAAGACTCCGGCAGCCTTGGCACTGAATCAGACGCAACACTGTAAACA
  GCTGGAGGGCCTGGTGTCTGCGCAGGTGCAGCTCTGCCGCAGCAACCTGGAGCTCATGCGCACCATCGTG
  CACGCCGCCCGGGGGGCCATGAAGGCCTGCCGTAGGGCCTTCGCTGACATGCGCTGGAACTGCTCCTCCA
  TCGAGCTCGCCCCCAACTACCTGCTTGACCTGGAGAGAGGTACACGGGAGTCAGCCTTCGTGTATGCCCT
  GTCGGCCGCCACCATCAGTCACACCATCGCCCGGGCCTGCACCTCTGGCGACCTGCCCGGCTGCTCCTGC
  GGCCCCGTCCCAGGTGAGCCACCCGGGCCCGGGAACCGCTGGGGAGGATGTGCGGACAACCTCAGCTACG
  GGCTCCTCATGGGGGCCAAGTTTTCCGATGCTCCTATGAAGGTGAAAAAAACAGGATCCCAAGCCAATAA
  ACTGATGCGTCTACACAACAGTGAAGTGGGGAGACAGGCTCTACGTGCCTCCCTGGAAACGAAGTGTAAA
  TGCCATGGGGTGTCTGGCTCCTGCTCCATCCGCACCTGTTGGAAGGGGCTGCAAGAGCTCCAGGACGTGG
  CTGCTGACCTCAAGACCCGCTACCTGTCAGCCACGAAGGTGGTACACCGGCCTATGGGCACCCGCAAACA
  CTTGGTGCCCAAGGACCTGGATATCCGGCCTGTGAAGGACTCAGAACTTGTGTATCTACAGAGCTCCCCT
  GACTTCTGCATGAAGAATGAGAAGGTGGGATCCCATGGGACCCAAGACAGGCAGTGCAACAAGACTTCCA
  ACGGCAGTGACAGCTGCGACCTCATGTGCTGTGGGCGCGGCTACAACCCCTACACGGACAGAGTGGTGGA
  GCGATGTCACTGCAAGTACCACTGGTGCTGCTACGTCACCTGCCGCAGGTGTGAGCGCACGGTGGAGCGC
  TACGTCTGCAAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_009519
• Insert Size: 1065 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC125482, AAI25483
• RefSeq Size: 1559 bp
• RefSeq ORF: 1065 bp
• Locus ID: 22411
• UniProt ID: P48615
• Cytogenetics: 7 53.9 cM
• Gene Summary: Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 both encode the same isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.