Strap (NM_011499) Mouse Untagged Clone

CAT#: MC209478

Strap (untagged) - Mouse serine/threonine kinase receptor associated protein (Strap), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Strap
• Synonyms: AW557906; C78091; C79202; Unrip
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC209478 representing NM_011499
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCATGAGGCAGACGCCGCTCACTTGCTCGGGCCACACGCGGCCCGTGGTGGATTTGGCCTTCAGCG
  GCATCACGCCTTACGGCTACTTTCTGATCAGCGCTTGCAAAGATGGCAAGCCCATGCTCCGCCAGGGAGA
  TACAGGAGACTGGATTGGAACATTTTTGGGTCATAAAGGTGCTGTTTGGGGTGCAACATTGAATAAGGAT
  GCCACCAAAGCTGCGACAGCAGCTGCAGACTTCACAGCCAAAGTATGGGATGCGGTCTCAGGAGATGAAT
  TGATGACCCTGGCTCATAAGCACATTGTCAAGACTGTGGATTTCACACAGGATAGCAATTACCTGCTAAC
  TGGGGGACAGGATAAACTGCTGCGCATATATGACTTGAACAAACCTGAAGCAGAACCTAAGGAAATCAGT
  GGCCACACTTCTGGTATTAAAAAGGCTCTGTGGTGCAGTGACGATAAACAGATCCTTTCAGCGGATGATA
  AAACTGTTCGGCTCTGGGATCATGCCACAATGACAGAAGTGAAATCTCTGAATTTTAATATGTCTGTTAG
  CAGCATGGAGTATATTCCTGAAGGAGAGATTTTGGTTATTACTTATGGACGATCTATTGCTTTTCATAGT
  GCAGTAAGTCTGGAGCCAATTAAATCCTTTGAAGCTCCTGCGACCATCAATTCTGCGTCTCTTCATCCAG
  AGAAGGAGTTTCTTGTTGCGGGTGGAGAAGACTTTAAACTGTACAAGTATGATTATAACAGTGGAGAAGA
  GTTAGAATCCTACAAAGGTCACTTTGGTCCCATTCACTGTGTGAGATTCAGTCCTGATGGGGAACTCTAT
  GCCAGCGGTTCTGAAGATGGGACATTGAGATTGTGGCAAACTGTGGTAGGAAAGACCTATGGCCTGTGGA
  AATGCGTGCTTCCTGAGGAAGACAGCGGGGAACTGGCAAAGCCAAAGATCGGATTTCCAGAAACAGCAGA
  GGAAGAGCTGGAAGAAATTGCTTCAGAGAATTCAGATTCCATCTATTCATCAACTCCTGAAGTTAAGGCC
  TGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_011499
• Insert Size: 1053 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011499.3, NP_035629.2
• RefSeq Size: 2644 bp
• RefSeq ORF: 1053 bp
• Locus ID: 20901
• UniProt ID: Q9Z1Z2
• Cytogenetics: 6 G1
• Gene Summary: The SMN complex plays a catalyst role in the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome. Thereby, plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP. In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. Dissociation by the SMN complex of CLNS1A from the trapped Sm proteins and their transfer to an SMN-Sm complex triggers the assembly of core snRNPs and their transport to the nucleus. STRAP plays a role in the cellular distribution of the SMN complex. Negatively regulates TGF-beta signaling but positively regulates the PDPK1 kinase activity by enhancing its autophosphorylation and by significantly reducing the association of PDPK1 with 14-3-3 protein (By similarity).[UniProtKB/Swiss-Prot Function]