Renbp (NM_023132) Mouse Untagged Clone

CAT#: MC209313

Renbp (untagged) - Mouse renin binding protein (Renbp), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Renbp
• Synonyms: Age; Rnbp
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC209313 representing NM_023132
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGGACCTAGGACTCCTAATGTTACAGGACATGGAGAAGGAACGGGAGACACTGCAGGTCTGGAAGA
  AGCGTGTGGAACAAGAGCTTGATCGTGTGATCGCTTTCTGGATGGAGCATTCCCATGACCAGGAACACGG
  GGGCTTCTTCACATGTCTTGGCCGTGATGGGAAGGTATATGATCACCTCAAATATGTTTGGCTGCAGGGG
  AGGCAGGTATGGATGTATTGTCGCTTATACCGCAGTTTTGAGCGCTTCCGCCGTGTTGAGCTTCTGGATG
  CAGCAAGAGCAGGTGGTGAATTTTTGCTGCGTTATGCCCGGGTGGCACCACCTGGCAAGAAATGTGCTTT
  TGTGCTGACTCGGGATGGCCGGCCAGTGAAGGTGCAGCGGACCATTTTCAGCGAGTGCTTCTACACCATG
  GCCATGAATGAACTGTGGAAAGTAACGGGGGAAGTGCGTTATCAGAGTGAAGCCATAGAGATGATGGATC
  AGATCATCCACTGGGTACGGGAGGACCCGGCTGGGTTGGGCCGGCCTCAGCTCTCAGGGGCACTGGCCAC
  AGAGCCCATGGCAGTGCCCATGATGCTGCTCAGCCTGGTGGAGCAGCTTGGAGAAGAAGATGAGGAGCTG
  ACCAACATGTATGCAGAACTAGGGGACTGGTGTGTCCACAGGATTCTTCAGCATGTCCAGAGGGATGGAC
  AAGTTGTACTGGAGAATGTATCAGAGGATGGAAAAGAGCTTCCTGGTTGCCTTGGAAGACATCAGAACCC
  AGGCCACACACTGGAAGCTGGCTGGTTTCTGCTCCAGTATGCCCTCAGGAAAGGTGACCCCAAACTTCGA
  ATGCACATCATTGACAAGTTTCTCTTATTGCCTTTCCACTCTGGATGGGACCCTGAACATGGAGGCCTCT
  TCTACTTCCAGGATGCGGATGGTCTCTGCCCTACCCAGCTGGAATGGAACATGAAGCTGTGGTGGCCACA
  CAGTGAAGCCATGATTGCCTTCCTCATGGGTTATAGTGACAGTGGGGACCCTGCCTTGCTGCATCTCTTC
  TACAAGGTGGCTGAGTACACCTTCCGCCAGTTTCGTGATCCTGAGTATGGGGAATGGTTTGGCTACCTGA
  ACCAAGAGGGAAAGGTGGCCCTCACCATCAAGGGAGGTCCTTTTAAAGGCTGCTTCCATGTGCCGCGGTG
  CCTGGCCATGTGCGAGCAGATTCTAGGAGCCCTACTCCAACGCCTTGAGCCCGCCCCCCTCGACTCCTCG
  CCCGCTGTCTCTACCCATGAAGGCTCGAAATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_023132
• Insert Size: 1293 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_023132.3, NP_075621.3
• RefSeq Size: 1434 bp
• RefSeq ORF: 1293 bp
• Locus ID: 19703
• UniProt ID: P82343
• Cytogenetics: X 37.49 cM
• Gene Summary: Catalyzes the interconversion of N-acetylglucosamine to N-acetylmannosamine. Binds to renin forming a protein complex called high molecular weight (HMW) renin and inhibits renin activity. Involved in the N-glycolylneuraminic acid (Neu5Gc) degradation pathway.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). CCDS Note: The coding region has been updated to extend the N-terminus to one that is more supported by the available transcript data. The updated start codon, which adds 1 aa to the protein, is only conserved in mouse and rat, and it has a weak Kozak signal. It is possible that ribosomal leaky scanning may allow the better conserved downstream start codon to be used some of the time. There is no experimental evidence indicating which start codon is preferentially used in vivo.