Ptafr (NM_001081211) Mouse Untagged Clone

CAT#: MC209250

Ptafr (untagged) - Mouse platelet-activating factor receptor (Ptafr), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ptafr
• Synonyms: P; PAFR
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC209250 representing NM_001081211
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCACAATGGCTCCTTTCGTGTGGATTCTGAGTTTCGATACACGCTCTTTCCGATTGTTTACAGTG
  TCATCTTTATACTGGGGGTGGTTGCCAACGGCTATGTGCTATGGGTCTTTGCTAACTTGTACCCTTCCAA
  GAAACTAAATGAGATAAAGATCTTTATGGTGAATCTCACTATGGCTGACCTGCTCTTCCTGATCACCCTC
  CCACTGTGGATTGTCTACTACTACAACGAGGGCGACTGGATTCTACCCAACTTCCTGTGCAACGTGGCTG
  GCTGCCTCTTCTTCATCAATACCTACTGCAGTGTGGCCTTTTTGGGTGTCATCACTTATAACCGCTACCA
  GGCAGTAGCCTATCCCATCAAGACTGCACAGGCCACCACCCGCAAGCGTGGCATCTCTTTGTCCCTGATC
  ATTTGGGTATCCATTGTGGCTACTGCATCCTATTTCCTGGCCACAGACTCCACCAACCTAGTGCCCAATA
  AGGATGGCTCAGGCAACATCACCCGCTGCTTTGAGCATTATGAGCCATACAGTGTGCCCATCCTTGTTGT
  TCATGTCTTCATCGCCTTCTGCTTCTTCCTCGTCTTCTTCCTTATCTTCTACTGCAACTTGGTCATCATC
  CACACGCTGCTCACGCAGCCCATGAGGCAGCAGCGCAAAGCGGGGGTGAAGCGGAGGGCGCTGTGGATGG
  TCTGCACGGTCTTGGCGGTATTCATCATCTGTTTTGTGCCCCATCACGTGGTCCAGCTGCCCTGGACCCT
  AGCAGAGTTGGGCTACCAGACCAACTTCCATCAGGCTATTAATGATGCCCATCAAATCACCCTCTGCCTC
  CTCAGCACCAACTGTGTCTTAGATCCCGTTATCTATTGCTTTCTTACCAAGAAGTTCCGAAAGCACCTCA
  GTGAGAAGTTTTACAGCATGCGCAGTAGCCGGAAGTGCTCCAGAGCCACGAGTGACACGTGCACCGAGGT
  GATAGTGCCAGCCAACCAGACTCCTATTGTGTCGCTGAAAAATTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001081211
• Insert Size: 1026 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001081211.2, NP_001074680.1
• RefSeq Size: 3660 bp
• RefSeq ORF: 1026 bp
• Locus ID: 19204
• UniProt ID: Q62035
• Cytogenetics: 4 65.56 cM
• Gene Summary: This gene encodes a member of the G-protein coupled receptor 1 family of proteins. Binding of the encoded protein to its phospholipid ligand, platelet-activating factor (PAF), may regulate the inflammatory response and the perception of pain. Homozygous knockout mice for this gene exhibit impaired anaphylactic response, resistance to bacterial infection, and reduced pain-related behavior. [provided by RefSeq, Aug 2015]