Pdx1 (NM_008814) Mouse Untagged Clone

CAT#: MC209160

Pdx1 (untagged) - Mouse pancreatic and duodenal homeobox 1 (Pdx1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pdx1
• Synonyms: IDX-1; IPF-1; Ipf1; Mody4; pdx-1; STF-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC209160 representing NM_008814
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACAGTGAGGAGCAGTACTACGCGGCCACACAGCTCTACAAGGACCCGTGCGCATTCCAGAGGGGCC
  CGGTGCCAGAGTTCAGCGCTAACCCCCCTGCGTGCCTGTACATGGGCCGCCAGCCCCCACCTCCGCCGCC
  ACCCCAGTTTACAAGCTCGCTGGGATCACTGGAGCAGGGAAGTCCTCCGGACATCTCCCCATACGAAGTG
  CCCCCGCTCGCCTCCGACGACCCGGCTGGCGCTCACCTCCACCACCACCTTCCAGCTCAGCTCGGGCTCG
  CCCATCCACCTCCCGGACCTTTCCCGAATGGAACCGAGCCTGGGGGCCTGGAAGAGCCCAACCGCGTCCA
  GCTCCCTTTCCCGTGGATGAAATCCACCAAAGCTCACGCGTGGAAAGGCCAGTGGGCAGGAGGTGCTTAC
  ACAGCGGAACCCGAGGAAAACAAGAGGACCCGTACTGCCTACACCCGGGCGCAGCTGCTGGAGCTGGAGA
  AGGAATTCTTATTTAACAAATACATCTCCCGGCCCCGCCGGGTGGAGCTGGCAGTGATGTTGAACTTGAC
  CGAGAGACACATCAAAATCTGGTTCCAAAACCGTCGCATGAAGTGGAAAAAAGAGGAAGATAAGAAACGT
  AGTAGCGGGACCCCGAGTGGGGGCGGTGGGGGCGAAGAGCCGGAGCAAGATTGTGCGGTGACCTCGGGCG
  AGGAGCTGCTGGCAGTGCCACCGCTGCCACCTCCCGGAGGTGCCGTGCCCCCAGGCGTCCCAGCTGCAGT
  CCGGGAGGGCCTACTGCCTTCGGGCCTTAGCGTGTCGCCACAGCCCTCCAGCATCGCGCCACTGCGACCG
  CAGGAACCCCGGTGA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_008814
• Insert Size: 855 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008814.3, NP_032840.1
• RefSeq Size: 1287 bp
• RefSeq ORF: 855 bp
• Locus ID: 18609
• UniProt ID: P52946
• Cytogenetics: 5 86.84 cM
• Gene Summary: Activates insulin and somatostatin gene transcription. Key regulator of islet peptide hormone expression but also responsible for the development of the pancreas, most probably by determining maturation and differentiation of common pancreatic precursor cells in the developing gut. As part of a PDX1:PBX1b:MEIS2b complex in pancreatic acinar cells is involved in the transcriptional activation of the ELA1 enhancer; the complex binds to the enhancer B element and cooperates with the transcription factor 1 complex (PTF1) bound to the enhancer A element. Binds the DNA sequence 5'-CC[CT]TAATGGG-3'.[UniProtKB/Swiss-Prot Function]