Nfic (NM_026756) Mouse Untagged Clone

CAT#: MC209026

Nfic (untagged) - Mouse nuclear factor I/C (Nfic), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Nfic
• Synonyms: 1110019L22Rik; 1500041O16Rik; AA589446; AI746521; NF1-C
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC209026 representing NM_026756
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTATTCCTCCCCGCTCTGCCTGACCCAGGATGAGTTCCACCCGTTCATCGAGGCGCTGCTGCCGCATG
  TGCGCGCCTTCGCCTACACCTGGTTCAACCTGCAGGCGCGCAAGCGCAAGTACTTCAAGAAGCACGAGAA
  GCGCATGTCCAAGGACGAGGAGCGCGCGGTGAAGGATGAGCTGCTGGGTGAAAAGGCCGAAGTGAAGCAG
  AAGTGGGCGTCGCGGCTGCTGGCCAAGCTGCGCAAGGACATCCGGCCCGAGTGCCGTGAAGACTTCGTAC
  TGGCTGTGACTGGCAAGAAGGCGCCAGGCTGTGTGCTGTCCAACCCGGACCAGAAGGGCAAGATGCGGCG
  CATCGACTGCCTGCGCCAGGCCGACAAAGTGTGGCGTCTGGACCTGGTCATGGTCATCCTGTTCAAGGGC
  ATCCCACTGGAGAGCACAGATGGTGAGCGGCTGGTGAAGGCGGCTGCCTGTGCGCACCCGGTGCTGTGTG
  TGCAGCCGCACCATATTGGCGTGGCCGTCAAGGAGCTGGACCTGTACCTGGCCTACTTTGTGCGGGAGCG
  CGATGCGGAGCAGAGCAGCAGCCCCCGGACAGGTGTGGGCTCAGACCAGGAGGACAGCAAGCCCATAACG
  CTGGACACCACAGACTTCCAGGAGAGCTTTGTCACGTCAGGTGTGTTCAGTGTCACGGAGCTGATCCAAG
  TGTCTCGGACACCTGTGGTGACCGGAACCGGACCCAACTTCTCACTGGGAGAGCTGCAGGGTCACCTGGC
  ATATGACCTGAACCCGGCCAGCGCCGGCATGAGAAGGACTCTACCCAGCACCTCCTCCAGTGGGAGCAAG
  CGGCACAAGTCGGGCTCGATGGAAGAGGACGTGGACACGAGCCCCGGCGGCGACTACTACACGTCGCCCA
  ACTCACCCACGAGTAGCAGCCGCAACTGGACGGAAGACATAGAAGGAGGCATCTCATCCCCGGTGAAGAA
  GACAGAGATGGACAAATCTCCATTCAACAGCCCGTCCCCCCAGGACTCCCCCCGGCTCTCCAGCTTCACA
  CAACACCACAGGCCGGTCATCGCGGTACACAGTGGCATCGCGCGGAGCCCCCACCCGACCTCGGCCCTGC
  ACTTCCCTGCCACGCCCATCCTGCCACAGACAGCCTCCACCTACTTCCCCCATACGGCCATCCGGTACCC
  GCCACATCTCAACCCCCAGGACCCGCTCAAGGATCTGGTGTCCCTGGCCTGTGACCCGGCCACCCAGCAG
  CCTGGACCGTCCTGGTACCTGGGGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_026756
• Insert Size: 1287 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_026756.2, NP_081032.2
• RefSeq Size: 6218 bp
• RefSeq ORF: 1287 bp
• Locus ID: 18029
• Cytogenetics: 10 39.72 cM
• Gene Summary: Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an alternate penultimate exon, resulting in a novel 3' coding region and shorter 3' UTR, compared to variant 1. The resulting isoform (b) has a shorter and distinct C-terminus, compared to isoform a.