Nck1 (NM_010878) Mouse Untagged Clone

CAT#: MC209014

Nck1 (untagged) - Mouse non-catalytic region of tyrosine kinase adaptor protein 1 (Nck1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Nck1
• Synonyms: 6330586M15Rik; D230010O13Rik; Nck; Nck-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC209014 representing NM_010878
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGAAGAAGTGGTGGTGGTGGCCAAATTTGATTATGTGGCACAGCAGGAACAAGAGCTGGATATCA
  AGAAGAATGAGCGATTATGGCTCCTGGATGACTCTAAATCCTGGTGGCGAGTTCGAAATTCCATGAATAA
  AACAGGTTTTGTCCCTTCTAACTATGTGGAAAGAAAAAACAGTGCTCGGAAAGCATCTATTGTTAAAAAC
  CTGAAGGACACCTTAGGTATTGGAAAAGTGAAAAGAAAACCCAGTGTTCCAGATACTGCATCTCCTGCTG
  ATGATAGCTTTGTTGATCCAGGAGAACGTCTCTATGACCTTAACATGCCTGCTTTTGTGAAATTTAACTA
  CATGGCTGAGAGAGAGGATGAGTTGTCATTGATAAAAGGGACCAAGGTGATCGTCATGGAGAAATGCAGT
  GATGGATGGTGGCGTGGCAGCTACAACGGACAAATTGGATGGTTTCCTTCAAACTATGTAACTGAAGAAG
  GTGACAGTCCTTTGGGTGATCATGTAGGTTCTCTGTCAGAGAAATTAGCAGCAGTTGTCAATAACCTAAA
  TACGGGTCAAGTATTGCATGTTGTACAGGCTCTTTACCCGTTTAGCTCATCCAATGATGAAGAACTCAAT
  TTTGAGAAAGGCGATGTAATGGATGTTATTGAAAAGCCGGAAAATGACCCAGAGTGGTGGAAATGCAGGA
  AAATCAATGGCATGGTTGGCCTGGTGCCAAAAAACTACGTTACCATTATGCAAAACAATCCATTAACCTC
  AGGTTTGGAACCATCTCCTCCACAATGTGATTACATTAGGCCTTCACTCACTGGGAAGTTTGCTGGCAAT
  CCTTGGTATTATGGCAAAGTCACCAGGCACCAGGCAGAAATGGCATTAAATGAAAGAGGGCATGAAGGAG
  ACTTCCTCATTCGTGACAGTGAATCTTCGCCAAATGATTTCTCAGTATCACTAAAAGCACAAGGGAAAAA
  CAAGCATTTTAAAGTCCAGCTGAAAGAGACTGTTTACTGCATTGGGCAGCGGAAATTCAGCACCATGGAG
  GAACTTGTAGAACATTACAAAAAGGCACCGATCTTTACAAGTGAGCAAGGAGAAAAATTATATCTCGTCA
  AGCATTTGTCTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_010878
• Insert Size: 1134 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010878.3, NP_035008.2
• RefSeq Size: 2467 bp
• RefSeq ORF: 1134 bp
• Locus ID: 17973
• UniProt ID: Q99M51
• Cytogenetics: 9 E3.3
• Gene Summary: Adapter protein which associates with tyrosine-phosphorylated growth factor receptors, such as KDR and PDGFRB, or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in the DNA damage response, not in the detection of the damage by ATM/ATR, but for efficient activation of downstream effectors, such as that of CHEK2. Plays a role in ELK1-dependent transcriptional activation in response to activated Ras signaling. Modulates the activation of EIF2AK2/PKR by dsRNA (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longer isoform (1).