Kcnk2 (NM_001159850) Mouse Untagged Clone

CAT#: MC208810

Kcnk2 (untagged) - Mouse potassium channel, subfamily K, member 2 (Kcnk2), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Kcnk2
• Synonyms: A430027H14Rik; AI848635; TREK-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC208810 representing NM_001159850
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTTGCCAGCGCCTCGCGGGAGAGACCCGGCTATACTGCAGGAGTGGCGGCCCCTGACTTGCTGGATC
  CCAAGTCTGCTGCTCAGAACTCCAAACCGAGGCTCTCATTCTCCTCAAAACCCACCGTGCTTGCTTCCCG
  GGTGGAGAGTGACTCGGCCATTAATGTTATGAAATGGAAGACAGTCTCCACGATTTTCCTGGTGGTCGTC
  CTCTACCTGATCATCGGAGCCACGGTGTTCAAGGCATTGGAGCAGCCTCAGGAGATTTCCCAGAGGACCA
  CCATTGTGATCCAGAAGCAGACCTTCATAGCCCAGCATGCCTGCGTCAACTCCACCGAGCTGGACGAACT
  CATCCAGCAAATAGTGGCAGCAATAAACGCAGGGATTATCCCCTTAGGAAACAGCTCCAATCAAGTTAGT
  CACTGGGACCTCGGAAGCTCTTTCTTCTTTGCTGGTACTGTTATCACAACCATAGGATTTGGAAACATCT
  CCCCACGAACTGAAGGTGGAAAAATATTCTGCATCATCTATGCCTTGCTGGGAATTCCCCTCTTTGGCTT
  TCTACTGGCTGGGGTTGGTGATCAGCTAGGAACTATATTTGGAAAAGGAATTGCCAAAGTGGAAGACACA
  TTTATTAAGTGGAATGTTAGTCAGACGAAGATTCGTATCATCTCCACCATCATCTTCATCCTGTTTGGCT
  GTGTCCTCTTTGTGGCTCTCCCTGCGGTCATATTCAAGCACATAGAAGGCTGGAGCGCCCTGGACGCTAT
  CTATTTTGTGGTTATCACTCTGACGACCATTGGATTTGGAGACTACGTGGCAGGTGGATCAGACATTGAA
  TATCTGGACTTCTACAAGCCTGTGGTGTGGTTCTGGATCCTCGTTGGGCTGGCCTACTTTGCAGCTGTTC
  TGAGCATGATTGGGGACTGGCTACGGGTGATCTCTAAGAAGACGAAGGAAGAGGTGGGAGAGTTCAGAGC
  GCATGCCGCTGAGTGGACAGCCAATGTCACGGCCGAGTTCAAGGAAACGAGGAGGCGGCTGAGCGTGGAG
  ATCTACGACAAGTTCCAGCGTGCCACATCCGTGAAGCGGAAGCTCTCCGCAGAGCTGGCGGGCAACCACA
  ACCAGGAACTGACTCCGTGTAGGAGGACCCTGTCTGTGAACCACCTGACCAGCGAGAGGGAAGTCCTGCC
  TCCCTTGCTGAAGGCTGAGAGCATCTATCTGAACGGTCTGACACCACACTGTGCTGGTGAGGACATAGCT
  GTCATTGAGAACATGAAGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001159850
• Insert Size: 1281 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001159850.1, NP_001153322.1
• RefSeq Size: 3423 bp
• RefSeq ORF: 1281 bp
• Locus ID: 16526
• UniProt ID: P97438
• Cytogenetics: 1 H6
• Gene Summary: Ion channel that contributes to passive transmembrane potassium transport. Reversibly converts between a voltage-insensitive potassium leak channel and a voltage-dependent outward rectifying potassium channel in a phosphorylation-dependent manner. In astrocytes, forms mostly heterodimeric potassium channels with KCNK1, with only a minor proportion of functional channels containing homodimeric KCNK2 (PubMed:24496152). In astrocytes, the heterodimer formed by KCNK1 and KCNK2 is required for rapid glutamate release in response to activation of G-protein coupled receptors, such as F2R and CNR1 (PubMed:24496152).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longest isoform (1).