Kcnj6 (NM_001025585) Mouse Untagged Clone

CAT#: MC208807

Kcnj6 (untagged) - Mouse potassium inwardly-rectifying channel, subfamily J, member 6 (Kcnj6), transcript variant Girk2B, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Kcnj6
• Synonyms: BIR1; GIRK2; KATP2; KCNJ7; Kir3.2; weaver; wv
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC208807 representing NM_001025585
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACAATGGCCAAGTTAACTGAATCCATGACTAACGTCTTGGAAGGCGATTCCATGGACCAGGATGTGG
  AAAGCCCAGTGGCCATTCACCAGCCAAAGTTGCCTAAGCAGGCCAGGGACGACCTGCCGAGACACATCAG
  CCGAGACAGGACCAAAAGGAAAATCCAGAGGTACGTGAGGAAGGATGGGAAGTGCAACGTTCACCACGGC
  AATGTGCGGGAGACGTACCGATACCTGACGGACATCTTCACCACCCTGGTGGACCTGAAGTGGAGATTCA
  ACCTGTTGATCTTTGTCATGGTCTACACAGTGACGTGGCTTTTCTTTGGGATGATCTGGTGGCTGATTGC
  GTACATCCGGGGAGATATGGACCACATAGAGGACCCCTCGTGGACTCCTTGTGTCACCAACCTCAACGGG
  TTTGTCTCTGCTTTTTTATTCTCCATAGAGACAGAAACCACCATCGGTTATGGCTACCGGGTCATCACGG
  ACAAGTGCCCTGAGGGGATTATTCTCCTCTTAATCCAGTCCGTGTTGGGGTCCATTGTCAACGCCTTCAT
  GGTAGGATGTATGTTTGTGAAAATATCCCAACCCAAGAAGAGGGCAGAGACCCTGGTCTTTTCCACCCAC
  GCGGTGATCTCCATGCGGGATGGGAAACTGTGCTTGATGTTCCGGGTGGGGGACTTGAGGAATTCTCACA
  TTGTGGAGGCATCCATCAGAGCCAAGTTGATCAAGTCCAAACAGACTTCAGAGGGGGAGTTTATTCCCCT
  CAACCAGACTGATATCAACGTGGGGTACTACACAGGGGACGACCGGCTCTTTCTGGTGTCACCATTGATT
  ATTAGCCATGAAATTAACCAACAGAGTCCCTTCTGGGAGATCTCCAAAGCGCAGCTGCCTAAAGAGGAAC
  TGGAGATTGTGGTCATCCTGGAGGGAATGGTGGAAGCCACAGGTAAGATGGGTTTCGCCCTGGGTTTTCT
  GTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001025585
• Insert Size: 984 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001025585.2, NP_001020756.1
• RefSeq Size: 2049 bp
• RefSeq ORF: 984 bp
• Locus ID: 16522
• UniProt ID: P48542
• Cytogenetics: 16 55.44 cM
• Gene Summary: This potassium channel is controlled by G proteins. It plays a role in granule cell differentiation, possibly via membrane hyperpolarization. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium.[UniProtKB/Swiss-Prot Function]