Gnai1 (NM_010305) Mouse Untagged Clone

CAT#: MC208563

Gnai1 (untagged) - Mouse guanine nucleotide binding protein (G protein), alpha inhibiting 1 (Gnai1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Gnai1
• Synonyms: AU046200; Gialpha1; Gnai-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC208563 representing NM_010305
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCTGCACATTGAGCGCTGAGGACAAGGCGGCCGTGGAGCGCAGCAAGATGATCGACCGCAACCTCC
  GGGAGGACGGCGAGAAGGCGGCGCGGGAGGTCAAGCTGCTGCTGCTGGGTGCTGGGGAATCTGGAAAGAG
  TACCATTGTGAAGCAGATGAAGATTATCCACGAAGCCGGCTACTCGGAAGAGGAGTGTAAGCAGTACAAG
  GCAGTGGTCTACAGCAACACTATCCAGTCCATCATTGCCATCATTAGAGCCATGGGGAGGTTGAAAATCG
  ACTTCGGAGACTCTGCTCGGGCGGATGATGCTCGCCAACTTTTCGTGCTTGCTGGGGCTGCAGAAGAAGG
  CTTTATGACTGCAGAGCTCGCCGGTGTCATAAAGAGACTGTGGAAAGACAGTGGTGTGCAAGCCTGCTTC
  AACAGATCCCGGGAGTACCAGCTGAACGATTCGGCAGCGTACTATCTGAATGACTTGGACAGAATAGCAC
  AGCCAAATTACATCCCAACTCAGCAGGATGTCCTCAGAACCAGAGTGAAGACCACAGGGATTGTGGAAAC
  CCACTTTACCTTCAAAGATCTTCATTTTAAAATGTTTGACGTGGGAGGTCAGAGGTCAGAGCGGAAGAAG
  TGGATCCACTGCTTTGAAGGGGTGACCGCCATCATCTTCTGTGTGGCCCTGAGTGACTATGACCTGGTTC
  TTGCTGAAGATGAAGAAATGAACCGTATGCACGAGAGCATGAAGCTGTTCGATAGCATCTGTAACAACAA
  GTGGTTTACAGACACGTCCATCATCCTTTTCCTCAACAAGAAGGACCTCTTCGAAGAAAAAATAAAAAAG
  AGCCCCCTCACGATATGCTACCCAGAATATGCAGGCTCAAACACATATGAAGAAGCGGCCGCGTATATTC
  AGTGTCAGTTTGAAGACCTCAATAAAAGGAAGGACACAAAGGAAATTTACACCCACTTCACGTGCGCCAC
  AGATACGAAGAACGTGCAGTTCGTGTTCGATGCTGTAACAGACGTCATCATAAAGAATAACCTAAAAGAC
  TGTGGTCTCTTCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_010305
• Insert Size: 1065 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC138862, AAI38863
• RefSeq Size: 3193 bp
• RefSeq ORF: 1065 bp
• Locus ID: 14677
• UniProt ID: B2RSH2
• Cytogenetics: 5 8.16 cM
• Gene Summary: Guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs) in numerous signaling cascades. The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state. Signaling by an activated GPCR promotes GDP release and GTP binding. The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal. Both GDP release and GTP hydrolysis are modulated by numerous regulatory proteins (By similarity). Signaling is mediated via effector proteins, such as adenylate cyclase. Inhibits adenylate cyclase activity, leading to decreased intracellular cAMP levels (By similarity). The inactive GDP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. Required for normal cytokinesis during mitosis. Required for cortical dynein-dynactin complex recruitment during metaphase (By similarity).[UniProtKB/Swiss-Prot Function]