Doc2b (NM_007873) Mouse Untagged Clone

CAT#: MC208404

Doc2b (untagged) - Mouse double C2, beta (Doc2b), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Doc2b
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC208404 representing NM_007873
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCCTCCGGCGGCGCGGGGAGAAGGCGACCATCAGCATCCAGGAGCATATGGCCATCGACGTGTGTC
  CCGGCCCCATTCGGCCTATCAAGCAGATCTCCGATTATTTTCCCCGCTTCCCGCGGGGCCTCCCCCCTAC
  CGCCGCGCCCCGCGCCCCCGCGCCCCCGGACGCCCCCGCGCGCTCTCCCGCAGCCAGCGCCAGCCCCCGC
  AGCCCCTCCGACGGCGCCCGCGACGACGACGAAGATGTGGACCAGCTCTTCGGAGCCTACGGAGCCAGCC
  CAGGCCCCAGCCCCGGCCCCAGCCCCGCGAGGCCGCCCGCCAAGCCCCCCGAGGACGAGCCGGACGTCGA
  CGGCTACGAGTCAGACGACTGCACCGCCCTGGGTACGCTGGACTTCAGTCTGCTCTATGACCAGGAGAAC
  AACGCACTGCACTGCACCATCAGCAAGGCCAAGGGCCTGAAGCCGATGGACCACAATGGACTGGCTGATC
  CCTACGTCAAACTACACCTGCTGCCTGGAGCCAGCAAGGCAAATAAGCTCAGAACAAAAACTCTTCGGAA
  CACCCTGAACCCCTCGTGGAACGAGACCCTCACTTATTACGGAATCACGGATGAGGACATGGTCCGAAAG
  ACCCTGAGGATCTCCGTGTGTGATGAGGACAAATTCCGCCACAATGAGTTCATTGGAGAGACTCGGGTGC
  CCCTGAAGAAGCTGAAGCCCAATCACACCAAGACATTCAGCATCTGCCTGGAGAAGCAGCTGCCGGTGGA
  CAAGGCAGAGGACAAGTCTCTGGAAGAGCGAGGCCGCATCCTCATCTCCCTCAAGTACAGCTCACAGAAG
  CAGGGCCTGCTGGTGGGCATCGTGCGCTGTGCACACCTGGCTGCCATGGATGCTAATGGCTACTCGGACC
  CCTATGTGAAAACATATCTGAAGCCAGATGTAGACAAGAAATCCAAGCATAAGACAGCAGTGAAGAAGAA
  AACACTAAACCCAGAATTCAATGAGGAATTCTGTTACGAGATCAAGCATGGAGACCTGGCCAAAAAGACT
  CTGGAGGTCACTGTCTGGGATTATGACATTGGAAAATCCAATGATTTCATCGGTGGTGTGGTTCTGGGCA
  TCAACGCCAAGGGCGAGCGCCTGAAGCACTGGTTTGACTGCTTGAAGAACAAGGACAAGAGGATTGAGCG
  TTGGCACACGCTCACCAATGAGCTCCCAGGGGCTGTACTCAGCGACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_007873
• Insert Size: 1239 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007873.3, NP_031899.2
• RefSeq Size: 5068 bp
• RefSeq ORF: 1239 bp
• Locus ID: 13447
• UniProt ID: P70169
• Cytogenetics: 11 B5
• Gene Summary: The protein encoded by this gene is a calcium sensor involved in glucose-stimulated insulin secretion, spontaneous neurotransmitter release, and enhanced SNARE-dependent vesicle fusion. [provided by RefSeq, Sep 2015]