Bmp4 (NM_007554) Mouse Untagged Clone

CAT#: MC208195

Bmp4 (untagged) - Mouse bone morphogenetic protein 4 (Bmp4), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Bmp4
• Synonyms: Bmp-; Bmp-4; Bmp2; Bmp2b; Bmp2b-; Bmp2b-1; Bmp2b1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC208195 representing NM_007554
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATTCCTGGTAACCGAATGCTGATGGTCGTTTTATTATGCCAAGTCCTGCTAGGAGGCGCGAGCCATG
  CTAGTTTGATACCTGAGACCGGGAAGAAAAAAGTCGCCGAGATTCAGGGCCACGCGGGAGGACGCCGCTC
  AGGGCAGAGCCATGAGCTCCTGCGGGACTTCGAGGCGACACTTCTACAGATGTTTGGGCTGCGCCGCCGT
  CCGCAGCCTAGCAAGAGCGCCGTCATTCCGGATTACATGAGGGATCTTTACCGGCTCCAGTCTGGGGAGG
  AGGAGGAGGAAGAGCAGAGCCAGGGAACCGGGCTTGAGTACCCGGAGCGTCCCGCCAGCCGAGCCAACAC
  TGTGAGGAGTTTCCATCACGAAGAACATCTGGAGAACATCCCAGGGACCAGTGAGAGCTCTGCTTTTCGT
  TTCCTCTTCAACCTCAGCAGCATCCCAGAAAATGAGGTGATCTCCTCGGCAGAGCTCCGGCTCTTTCGGG
  AGCAGGTGGACCAGGGCCCTGACTGGGAACAGGGCTTCCACCGTATAAACATTTATGAGGTTATGAAGCC
  CCCAGCAGAAATGGTTCCTGGACACCTCATCACACGACTACTGGACACCAGACTAGTCCATCACAATGTG
  ACACGGTGGGAAACTTTCGATGTGAGCCCTGCAGTCCTTCGCTGGACCCGGGAAAAGCAACCCAATTATG
  GGCTGGCCATTGAGGTGACTCACCTCCACCAGACACGGACCCACCAGGGCCAGCATGTCAGAATCAGCCG
  ATCGTTACCTCAAGGGAGTGGAGATTGGGCCCAACTCCGGCCCCTCCTGGTCACTTTTGGCCATGATGGC
  CGGGGCCATACCTTGACCCGCAGGAGGGCCAAACGTAGTCCCAAGCATCACCCACAGCGGTCCAGGAAGA
  AGAATAAGAACTGCCGTCGCCATTCACTATACGTGGACTTCAGTGACGTGGGCTGGAATGATTGGATTGT
  GGCCCCACCCGGCTACCAGGCCTTCTACTGCCATGGGGACTGTCCCTTTCCACTGGCTGATCACCTCAAC
  TCAACCAACCATGCCATTGTGCAGACCCTAGTCAACTCTGTTAATTCTAGTATCCCTAAGGCCTGTTGTG
  TCCCCACTGAACTGAGTGCCATTTCCATGTTGTACCTGGATGAGTATGACAAGGTGGTGTTGAAAAATTA
  TCAGGAGATGGTGGTAGAGGGGTGTGGATGCCGCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_007554
• Insert Size: 1227 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC013459, AAH13459
• RefSeq Size: 1994 bp
• RefSeq ORF: 1227 bp
• Locus ID: 12159
• UniProt ID: P21275
• Cytogenetics: 14 23.95 cM
• Gene Summary: This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein regulates heart development and adipogenesis. Homozygous knockout mice die in utero, while a conditional knockout mouse exhibits defects in heart development. Transgenic mice overexpressing this gene in a neuron-specific manner exhibit a phenotype resembling the rare hereditary connective tissue disease, fibrodysplasia ossificans progressiva. [provided by RefSeq, Jul 2016]
  Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein.