Acadl (NM_007381) Mouse Untagged Clone

CAT#: MC208097

Acadl (untagged) - Mouse acyl-Coenzyme A dehydrogenase, long-chain (Acadl), nuclear gene encoding mitochondrial protein, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Acadl
• Synonyms: AA960361; AU018452; C79855; LC; LCAD
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC208097 representing NM_007381
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCGCGCCTGCTCCTCCGCTCCCTGCGCGTCCTGAGCGCCCGCTCGGCGCCACGCCCGCTGCCCT
  CCGCCCGATGTTCTCATTCTGGAGCAGAAGCGCGTCTAGAAACTCCTTCTGCTAAAAAATTAACTGACAT
  TGGAATCAGGAGAATCTTTTCCTCGGAGCATGACATTTTCCGGGAGAGTGTAAGGAAGTTTTTCCAAGAA
  GAAGTGATTCCTCACCACACAGAATGGGAGAAAGCTGGAGAAGTGAGTAGAGAGGTCTGGGAAAAAGCTG
  GCAAGCAGGGCTTGCTTGGCATCAACATCGCAGAGAAACATGGCGGCATTGGTGGGGACTTGCTCTCAAC
  AGCAGTTACTTGGGAAGAGCAAGCGTACTCCAATTGCACAGGCCCTGGCTTCAGCCTCCACTCAGATATT
  GTCATGCCCTATATTGCGAATTACGGCACAAAAGAACAGATCGAGAAGTTCATCCCCCAGATGACGGCGG
  GCAAGTGTATCGGTGCCATAGCCATGACAGAGCCTGGGGCTGGAAGTGACTTACAAGGAGTAAGAACGAA
  CGCCAAAAGATCTGGGAGTGATTGGATTCTCAATGGAAGCAAGGTGTTCATCACTAATGGCTGGTTAAGT
  GATCTCGTGATCGTCGTGGCCGTCACCAACCGTGAAGCTCGATCGCCTGCCCATGGCATTAGCCTCTTTT
  TGGTGGAAAACGGAATGAAAGGATTTATCAAGGGCCGGAAGCTGCATAAGATGGGAATGAAAGCTCAGGA
  CACAGCAGAACTATTCTTTGAAGATGTCCGATTGCCAGCTAATGCCTTACTTGGAGAAGAGAATAAAGGC
  TTCTACTACCTCATGCAAGAGCTTCCACAGGAAAGGCTCTTAATTGCTGAGTTGGCGATTTCTGCCTGTG
  AGTTCATGTTTGAAGAAACCAGGAACTACGTGAAGCAAAGAAAAGCTTTTGGGAAAACAGTTGCACACAT
  ACAGACGGTGCAGCATAAACTAGCAGAATTAAAGACACACATATGTGTCACCAGAGCTTTTGTGGACAGC
  TGTCTGCAGTTGCATGAAACCAAACGTCTGGACTCCGGTTCTGCTTCCATGGCAAAATACTGGGCATCTG
  AATTACAAAACAGTGTAGCTTATGAATGTGTGCAACTCCACGGAGGCTGGGGGTACATGTGGGAGTACCC
  GATTGCAAAAGCTTACGTGGATGCTCGGGTGCAGCCGATCTACGGTGGTACCAACGAAATAATGAAAGAG
  CTGATCGCAAGACAGATCGTCAGTGACAGCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_007381
• Insert Size: 1293 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007381.4, NP_031407.2
• RefSeq Size: 1948 bp
• RefSeq ORF: 1293 bp
• Locus ID: 11363
• UniProt ID: P51174
• Cytogenetics: 1 33.64 cM
• Gene Summary: This gene encodes a homotetrameric mitochondrial flavoprotein and is a member of the acyl-CoA dehydrogenase family. Members of this family catalyze the first step of fatty acid beta-oxidation, forming a C2-C3 trans-double bond in a FAD-dependent reaction. As beta-oxidation cycles through its four steps, each member of the acyl-CoA dehydrogenase family works at an optimum fatty acid chain-length. This enzyme has its optimum length between C12- and C16-acylCoA. In mice, deficiency of this gene can cause sudden death, cardiomyopathy as well as fasting and cold intolerance. [provided by RefSeq, Nov 2012]