Mafa (NM_194350) Mouse Untagged Clone

CAT#: MC208090

Mafa (untagged) - Mouse v-maf musculoaponeurotic fibrosarcoma oncogene family, protein A (avian) (Mafa), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mafa
• Synonyms: RIPE3b1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC208090 representing NM_194350
  Red=Cloning site Blue=ORF
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGCGGAGCTGGCGATGGGCGCAGAGCTGCCCAGCAGCCCACTGGCCATCGAGTACGTCAACGACT
  TCGACCTGATGAAGTTCGAGGTGAAGAAGGAGCCGCCCGAGGCCGAGCGCTTCTGCCACCGCCTGCCGCC
  CGGCTCGCTGTCCTCGACGCCCCTCAGCACGCCCTGCTCCTCGGTGCCCTCTTCGCCCAGCTTCTGCGCA
  CCCAGCCCGGGCACAGGCGGCGGCGCGGGCGGCGGGGGCAGCGCGGCTCAGGCCGGGGGCGCCCCGGGGC
  CGCCGAGTGGAGGCCCCGGCACTGTCGGGGGCGCCTCAGGAAAAGCGGTGCTGGAGGATCTGTACTGGAT
  GAGCGGGTACCAGCACCACCTGAACCCCGAGGCGCTCAACCTGACGCCGGAGGACGCGGTGGAGGCGCTC
  ATCGGCAGCGGCCACCACGGCGCGCACCACGGCGCGCATCACCCGGCGGCTGCTGCGGCCTATGAGGCCT
  TCCGGGGTCAGAGCTTCGCGGGCGGCGGCGGCGCGGACGACATGGGTGCCGGCCACCACCACGGCGCACA
  CCACACTGCCCACCATCATCACTCTGCCCACCATCACCATCACCACCATCACCACCACGGAGGCTCTGGC
  CACCACGGCGGAGGCGCGGGTCACGGCGGAGGCGGCGCAGGCCACCACGTGCGCTTGGAGGAGCGCTTCT
  CCGACGACCAGCTGGTATCCATGTCCGTGCGGGAGCTGAACCGGCAGCTCCGCGGCTTCAGCAAGGAGGA
  GGTCATCCGACTGAAACAGAAGCGGCGCACGCTCAAGAACCGCGGCTACGCGCAGTCGTGCCGCTTCAAG
  CGGGTGCAGCAGCGGCACATTCTGGAGAGCGAGAAGTGCCAGCTCCAGAGCCAGGTGGAGCAGCTGAAGC
  TGGAGGTGGGGCGTCTGGCCAAGGAGCGGGACCTGTACAAGGAGAAATACGAGAAGTTGGCGGGCCGGGG
  CGGCCCCGGGGGCGCGGGCGGGGCCGGCTTCCCTCGGGAGCCCTCGCCAGCGCAGGCTGGCCCCGGGGCG
  GCCAAAGGCGCACCCGACTTCTTTCTGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_194350
• Insert Size: 1080 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_194350.1, NP_919331.1
• RefSeq Size: 1080 bp
• RefSeq ORF: 1080 bp
• Locus ID: 378435
• UniProt ID: Q8CF90
• Cytogenetics: 15 D3
• Gene Summary: Transcriptional factor that activates insulin gene expression (PubMed:12368292, PubMed:15665000). Acts synergistically with NEUROD1/BETA2 and PDX1 (PubMed:15665000). Binds the insulin enhancer C1/RIPE3b element (PubMed:12917329, PubMed:14680841, PubMed:14973194, PubMed:15665000). Binds to consensus TRE-type MARE 5'-TGCTGACTCAGCA-3' DNA sequence (By similarity).[UniProtKB/Swiss-Prot Function]