Creb1 (NM_009952) Mouse Untagged Clone

CAT#: MC208007

Creb1 (untagged) - Mouse cAMP responsive element binding protein 1 (Creb1), transcript variant B, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Creb1
• Synonyms: 2310001E10Rik; 3526402H21Rik; AV083133; Creb; Creb-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC208007 representing NM_009952
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCATGGAATCTGGAGCAGACAACCAGCAGAGTGGAGATGCTGCTGTAACAGAAGCTGAAAATCAAC
  AAATGACAGTTCAAGCCCAGCCACAGATTGCCACATTAGCCCAGGTATCCATGCCAGCAGCTCATGCAAC
  ATCATCTGCTCCCACTGTAACCTTAGTGCAGCTGCCCAATGGGCAGACAGTCCAGGTCCATGGCGTTATC
  CAGGCGGCCCAGCCATCAGTTATCCAGTCTCCACAAGTCCAAACAGTTCAGTCTTCCTGTAAGGACTTAA
  AAAGACTTTTCTCCGGAACTCAGATTTCAACTATTGCAGAAAGTGAAGATTCACAGGAGTCTGTGGATAG
  TGTAACTGATTCCCAAAAACGAAGGGAAATCCTTTCAAGGAGGCCTTCCTACAGGAAAATTTTGAATGAC
  TTATCTTCTGATGCACCAGGGGTGCCAAGGATTGAAGAAGAAAAGTCAGAAGAGGAGACTTCAGCCCCTG
  CCATCACCACTGTAACAGTGCCAACCCCCATTTACCAAACTAGCAGTGGGCAGTACATTGCCATTACCCA
  GGGAGGAGCAATACAGCTGGCTAACAATGGTACGGATGGGGTACAGGGCCTGCAGACATTAACCATGACC
  AATGCAGCTGCCACTCAGCCGGGTACTACCATTCTACAGTATGCACAGACCACTGATGGACAGCAGATTC
  TAGTGCCCAGCAACCAAGTTGTTGTTCAAGCTGCCTCAGGCGATGTACAAACATACCAGATCCGCACAGC
  ACCCACGAGCACCATTGCCCCTGGAGTTGTTATGGCGTCCTCCCCAGCACTTCCTACACAGCCTGCTGAA
  GAAGCAGCACGGAAGAGAGAGGTCCGTCTAATGAAGAACAGGGAGGCAGCAAGAGAATGTCGTAGAAAGA
  AGAAAGAATATGTGAAATGTTTAGAGAACAGAGTGGCAGTGCTTGAAAACCAAAACAAAACATTGATTGA
  GGAGCTAAAAGCACTTAAGGACCTTTACTGCCACAAATCAGATTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_009952
• Insert Size: 1026 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009952.2, NP_034082.1
• RefSeq Size: 8431 bp
• RefSeq ORF: 1026 bp
• Locus ID: 12912
• UniProt ID: Q01147
• Cytogenetics: 1 32.74 cM
• Gene Summary: Phosphorylation-dependent transcription factor that stimulates transcription upon binding to the DNA cAMP response element (CRE), a sequence present in many viral and cellular promoters. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Involved in different cellular processes including the synchronization of circadian rhythmicity and the differentiation of adipose cells.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (B) includes an alternate in-frame exon in the 5' coding region, compared to variant A, resulting in a longer protein (isoform B), compared to isoform A. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.