Acot8 (NM_133240) Mouse Untagged Clone
CAT#: MC207854
Acot8 (untagged) - Mouse acyl-CoA thioesterase 8 (Acot8), (10ug)
"NM_133240" in other vectors (4)
Product Images
Specifications
Product Data | |
Type | Mouse Untagged Clone |
Tag | Tag Free |
Symbol | Acot8 |
Synonyms | PTE-2; Pte1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MC207854 representing NM_133240
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCAGCGCCAGAGGGTCTGGGAGATGCTCATGGCGACGCCGACCGCGGCGACCTTTCCGGGGACCTCC GTAGTGTGCTGGTCACGAGCGTGCTCAACCTCGAGCCGCTAGATGAAGATCTCTACAGAGGAAGGCATTA CTGGGTACCTACCTCCCAGCGGCTCTTTGGGGGTCAAATTATGGGCCAGGCCCTGGTGGCTGCAGCCAAG TCTGTGAGTGAAGACGTCCATGTCCACTCCCTGCACTGCTACTTTGTCCGGGCAGGGGACCCGAAAGTGC CAGTGCTGTACCACGTAGAGAGGATACGGACAGGAGCCAGCTTCTCAGTGCGCGCCGTGAAGGCTGTGCA GCATGGCAAGGCCATCTTCATCTGCCAGGCCTCCTTCCAGCAGATGCAGCCCAGCCCGCTGCAGCACCAG TTCTCCATGCCCTCCGTGCCCCCGCCAGAAGACCTGCTGGATCACGAGGCCCTCATTGACCAGTACTTAA GGGACCCTAACCTTCACAAGAAGTATCGAGTGGGGCTGAACCGAGTTGCTGCCCAGGAGGTACCTATTGA GATCAAGGTGGTGAACCCACCCACCCTGACCCAGCTGCAGGCACTGGAGCCCAAACAGATGTTCTGGGTG CGTGCCCGGGGCTACATTGGGGAAGGTGACATCAAGATGCATTGCTGTGTGGCTGCTTATATCTCTGACT ACGCCTTCCTGGGTACAGCACTGCTGCCCCACCAGTCCAAGTATAAGGTGAACTTCATGGCGTCACTGGA TCACTCCATGTGGTTTCATGCCCCATTCCGAGCCGACCACTGGATGCTGTACGAGTGTGAGAGCCCCTGG GCTGGTGGCTCTCGAGGGCTGGTGCATGGGCGGCTGTGGCGTCGGGATGGGGTCCTTGCTGTGACCTGTG CCCAGGAGGGTGTGATCCGATTGAAGCCTCAGGTGTCAGAGAGTAAGCTATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_133240 |
Insert Size | 963 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_133240.2, NP_573503.2 |
RefSeq Size | 1161 bp |
RefSeq ORF | 963 bp |
Locus ID | 170789 |
UniProt ID | P58137 |
Cytogenetics | 2 H3 |
Gene Summary | Acyl-coenzyme A (acyl-CoA) thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH (PubMed:11673457). Acyl-coenzyme A thioesterase 8/ACOT8 display no strong substrate specificity with respect to the carboxylic acid moiety of Acyl-CoAs (PubMed:11673457). Hydrolyzes medium length (C2 to C20) straight-chain, saturated and unsaturated acyl-CoAS but is inactive towards substrates with longer aliphatic chains (PubMed:11673457). Moreover, it catalyzes the hydrolysis of CoA esters of bile acids, such as choloyl-CoA and chenodeoxycholoyl-CoA and competes with bile acid CoA:amino acid N-acyltransferase (BAAT) (PubMed:11673457). ACOT8 is also able to hydrolyze CoA esters of dicarboxylic acids (PubMed:16141203). It is involved in the metabolic regulation of peroxisome proliferation (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). |
Documents
Product Manuals |
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Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG204622 | Acot8 (tGFP-tagged) - Mouse acyl-CoA thioesterase 8 (Acot8) |
USD 500.00 |
|
MR204622 | Acot8 (Myc-DDK-tagged) - Mouse acyl-CoA thioesterase 8 (Acot8) |
USD 300.00 |
|
MR204622L3 | Lenti ORF clone of Acot8 (Myc-DDK-tagged) - Mouse acyl-CoA thioesterase 8 (Acot8) |
USD 600.00 |
|
MR204622L4 | Lenti ORF clone of Acot8 (mGFP-tagged) - Mouse acyl-CoA thioesterase 8 (Acot8) |
USD 600.00 |
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